Seasonal differences and protection by creatine or arginine pretreatment in ischemia of mammalian and molluscan neurons in vitro

Brain Research
Tatyana A ZaparaAleksander S Ratushniak

Abstract

We investigated the dose-response relationship of protection by creatine against ischemic damage, and we asked whether or not such protection may be observed in invertebrate neurons that might provide a simpler experimental model. Rat isolated pyramidal neurons from the CA3 region of hippocampus subjected to ischemia ("in vitro ischemia") showed anoxic depolarization (AD) after 3-7 min of incubation in anoxic medium. Membrane potential (MP) was reduced 25-78% from preanoxic value. Inward current was decreased by an average 49%. Supplementation with creatine protected against these changes, with 1 mM being the minimal effective concentration, 2 mM providing a near-maximal protection, a maximal effect being obtained with 5 mM creatine. No additional protection was provided by up to 20 mM creatine. Isolated giant neurons of Lymnaea stagnalis showed a similar response to in vitro ischemia. However, a clear seasonal dependence of sensitivity of these cells was detected. In cells obtained during summertime (May-August), AD latency ranged from 3 to 10 min; during wintertime (December-March), this response did not occur even after 25-50 min. The addition of creatine to the medium did not cause changes in AD latency, probably because th...Continue Reading

References

Sep 1, 1992·European Journal of Biochemistry·V P Skulachev
Jul 1, 1991·Journal of Neuroscience Methods·V S Vorobjev
Feb 3, 1988·Neuroscience Letters·F A Edwards, P W Gage
Dec 1, 1981·Journal of Neurochemistry·T S Whittingham, P Lipton
Nov 1, 1982·The Journal of Physiology·I S Kass, P Lipton
Oct 14, 1994·Brain Research. Developmental Brain Research·J Y YagerB H Juurlink
Feb 20, 1998·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·K U Frerichs, J M Hallenbeck
Mar 21, 1998·The Journal of Experimental Zoology·G BernocchiM B Pisu

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Citations

Apr 24, 2012·Neurobiology of Disease·Hua Shen, Mark P Goldberg
Dec 3, 2011·Medicinal Research Reviews·Luisa PerassoMaurizio Balestrino
Jun 3, 2015·Molecular Neurobiology·Silvia RaveraIsabella Panfoli
Oct 28, 2016·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Leslie T BuckAqsa Malik
Oct 18, 2007·Neurochemical Research·Luisa PerassoMaurizio Balestrino
Dec 24, 2005·Alternatives to Laboratory Animals : ATLA·Rüdiger KöhlingMaurizio Balestrino
Mar 17, 2010·The Journal of Experimental Biology·Aqsa Malik, Leslie Thomas Buck

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