Seasonal variability in the persistence of dissolved environmental DNA (eDNA) in a marine system: The role of microbial nutrient limitation

PloS One
Ian Salter

Abstract

Environmental DNA (eDNA) can be defined as the DNA pool recovered from an environmental sample that includes both extracellular and intracellular DNA. There has been a significant increase in the number of recent studies that have demonstrated the possibility to detect macroorganisms using eDNA. Despite the enormous potential of eDNA to serve as a biomonitoring and conservation tool in aquatic systems, there remain some important limitations concerning its application. One significant factor is the variable persistence of eDNA over natural environmental gradients, which imposes a critical constraint on the temporal and spatial scales of species detection. In the present study, a radiotracer bioassay approach was used to quantify the kinetic parameters of dissolved eDNA (d-eDNA), a component of extracellular DNA, over an annual cycle in the coastal Northwest Mediterranean. Significant seasonal variability in the biological uptake and turnover of d-eDNA was observed, the latter ranging from several hours to over one month. Maximum uptake rates of d-eDNA occurred in summer during a period of intense phosphate limitation (turnover <5 hrs). Corresponding increases in bacterial production and uptake of adenosine triphosphate (ATP) de...Continue Reading

References

Jul 1, 1987·Journal of Bacteriology·M AlbanoD Dubnau
Aug 11, 1987·Nucleic Acids Research·C P Hodgson, R Z Fisk
Jan 1, 1987·Applied and Environmental Microbiology·J H PaulM F DeFlaun
May 2, 2001·Nature Reviews. Genetics·M HofreiterS Pääbo
Oct 31, 2001·Journal of Photochemistry and Photobiology. B, Biology·J L RavanatJ Cadet
Mar 1, 1988·Applied and Environmental Microbiology·John H PaulAndrew W David
Jul 1, 1988·Applied and Environmental Microbiology·J H PaulW H Jeffrey
Jul 1, 1989·Applied and Environmental Microbiology·J H PaulL H Cazares
Jul 20, 2007·Environmental Microbiology·Mikhail V ZubkovPeter H Burkill
Oct 27, 2007·Environmental Biosafety Research·Kaare M NielsenDaniele Daffonchio
Apr 11, 2008·Biology Letters·Gentile Francesco FicetolaPierre Taberlet
Jun 12, 2008·Conservation Biology : the Journal of the Society for Conservation Biology·Francesco FerrettiHeike K Lotze
Mar 3, 2011·PloS One·Enric SalaEnric Ballesteros
Sep 3, 2011·Environmental Microbiology·Hyatt C GreenKatharine G Field
Dec 14, 2011·Molecular Ecology·Philip Francis ThomsenEske Willerslev
Apr 11, 2012·Molecular Ecology·Pierre TaberletEske Willerslev
May 9, 2012·PloS One·Teruhiko TakaharaZen'ichiro Kawabata
Sep 7, 2012·PloS One·Philip Francis ThomsenEske Willerslev
Sep 17, 2013·Molecular Ecology Resources·David S PilliodLisette P Waits
Jan 16, 2014·Environmental Science & Technology·Matthew A BarnesDavid M Lodge
Jan 24, 2014·PloS One·Ryan P KellyLarry B Crowder
Apr 8, 2014·Current Biology : CB·Ramiro LogaresRamon Massana
May 14, 2014·Trends in Ecology & Evolution·Kristine BohmannMark de Bruyn
Nov 26, 2014·Proceedings of the National Academy of Sciences of the United States of America·Michael W LomasAdam C Martiny
Jun 3, 2015·Environmental Microbiology·Kimberly J Popendorf, Solange Duhamel
Jan 16, 2016·Environmental Science & Technology·Jessica EichmillerPeter W Sorensen
Mar 8, 2016·Molecular Ecology Resources·Anaïs Lacoursière-RousselLouis Bernatchez
Sep 1, 2016·Environmental Science & Technology·Lauren M SassoubreAlexandria B Boehm
Apr 26, 2017·PloS One·Elizabeth A AndruszkiewiczAlexandria B Boehm
Aug 16, 2017·Nature Ecology & Evolution·Eva Egelyng SigsgaardPhilip Francis Thomsen

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Citations

Nov 13, 2018·Communications Biology·Rupert A CollinsMartin J Genner
Nov 21, 2019·Proceedings. Biological Sciences·Jori B HarrisonSean M Rogers
Jul 16, 2021·The Science of the Total Environment·Mohan AmarasiriKazunari Sei
Jul 26, 2021·The Science of the Total Environment·Beilun ZhaoKrijn Trimbos

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