Seaweed polysaccharides as macromolecular crowding agents.

International Journal of Biological Macromolecules
Andrea De PieriDimitrios I Zeugolis

Abstract

Development of mesenchymal stem cell-based tissue engineered implantable devices requires prolonged in vitro culture for the development of a three-dimensional implantable device, which leads to phenotypic drift, thus hindering the clinical translation and commercialisation of such approaches. Macromolecular crowding, a biophysical phenomenon based on the principles of excluded-volume effect, dramatically accelerates and increases extracellular matrix deposition during in vitro culture. However, the optimal macromolecular crowder is still elusive. Herein, we evaluated the biophysical properties of various concentrations of different seaweed in origin sulphated polysaccharides and their effect on human adipose derived stem cell cultures. Carrageenan, possibly due to its high sulphation degree, exhibited the highest negative charge values. No correlation was observed between the different concentrations of the crowders and charge, polydispersity index, hydrodynamic radius and fraction volume occupancy across all crowders. None of the crowders, but arabinogalactan, negatively affected cell viability. Carrageenan, fucoidan, galactofucan and ulvan increased extracellular matrix (especially collagen type I and collagen type V) deposi...Continue Reading

References

Aug 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·B Goldberg
Apr 1, 1996·Journal of Biomedical Materials Research·D LogeartP Kern
Mar 4, 1997·Proceedings of the National Academy of Sciences of the United States of America·X LiuR Jaenisch
Apr 1, 1997·Molecular and Cellular Biology·V LefebvreB de Crombrugghe
Jun 1, 1997·Nature Genetics·D M BellK S Cheah
Aug 1, 1997·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Q ZhaoB De Crombrugghe
Feb 4, 1999·European Journal of Cell Biology·J L GirauxA M Fischer
May 13, 1999·Nature Genetics·W BiB de Crombrugghe
Sep 26, 2002·Thrombosis Research·Sabine MatouAnne-Marie Fischer
Aug 13, 2003·The Journal of Biological Chemistry·Takatora TakadaRyutaro Kamijo
Oct 7, 2003·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Yu PengzhanLi Pengcheng
May 15, 2004·Progress in Biophysics and Molecular Biology·S Schnell, T E Turner
Jun 5, 2007·Biochemical and Biophysical Research Communications·Natsuko KakudoKenji Kusumoto
Mar 12, 2008·Biochimie·Daisuke Miyoshi, Naoki Sugimoto
Dec 11, 2008·Marine Biotechnology·Mi-Ja KimJin-Sil Lee
Jun 16, 2010·The Journal of Biological Chemistry·Ling LingSimon M Cool
Aug 7, 2010·PloS One·Douglas TsaoNikolay V Dokholyan
Dec 14, 2011·Cytotherapy·Anne SchellenbergWolfgang Wagner
Sep 14, 2012·Phytotherapy Research : PTR·Anabela AlvesRui L Reis
Oct 25, 2012·Development·Yoshiki SasaiHidetaka Suga
Mar 21, 2013·Nature Communications·Barak AkabayovCharles C Richardson
Aug 15, 2013·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Anna Jean Wirth, Martin Gruebele
Oct 30, 2013·Angewandte Chemie·Shuntaro Takahashi, Naoki Sugimoto
Feb 6, 2014·ACS Applied Materials & Interfaces·Mamoni DashFederica Chiellini
Feb 18, 2014·Carbohydrate Polymers·Liang LiShirui Mao
Apr 16, 2014·Scientific Reports·Eva BrauchleKatja Schenke-Layland
May 23, 2014·Proceedings of the National Academy of Sciences of the United States of America·Nicholas F DupuisDavid J Nesbitt
Sep 2, 2014·The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology·Yu Seok SongDong-Seok Kim
Sep 12, 2014·Journal of Orthopaedic Science : Official Journal of the Japanese Orthopaedic Association·Daisuke KawamuraNorimasa Iwasaki

❮ Previous
Next ❯

Citations

Jan 23, 2021·International Journal of Molecular Sciences·Anna Di VitoGiuseppe Donato
Feb 14, 2021·International Journal of Pharmaceutics·Phuong H L TranThao T D Tran
Mar 31, 2021·Npj Regenerative Medicine·Andrea De PieriDimitrios I Zeugolis
Apr 15, 2021·Advanced Biology·Andrea De PieriKarin Wuertz-Kozak
May 1, 2021·Cells·Sergio Garnica-GalvezDimitrios I Zeugolis
Mar 4, 2021·American Journal of Physiology. Cell Physiology·Dimitrios I Zeugolis

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.