PMID: 6162901Apr 1, 1981Paper

Sebaceous gland differentiation: III. The uses and limitations of freshly isolated mouse preputial gland cells for the in vitro study of hormone and drug action

The Journal of Investigative Dermatology
V R Wheatley, J L Brind

Abstract

The effects of selected hormones and drugs on freshly isolated mouse preputial gland cells have been studied. The steroid hormones, testosterone, DHT, androstanediol, androsterone and androstanedione all failed to stimulate, and estradiol failed to inhibit, either DNA or lipid synthesis under the conditions studied. Thyroxine and insulin had no effect on lipogenesis but epinephrine and PGE2 caused significant stimulation as did Bt2cAMP. The antilipemic drugs clofibrate, nicotinic acid and hydroxycitrate were all able to inhibit lipogenesis. Of the anti-acne drugs only L-DOPA was able to inhibit lipogenesis, neither tetracycline nor trans-retinoic acid showed any effect. Pyridoxine was unable to inhibit lipogenesis but DMSO caused dramatic stimulation though it was without effect on DNA synthesis. Evidence is presented which suggests that the lack of response to steroid hormones is not due to the inability of the cells to take up and metabolize the steroids but is due to the fact that the time-span of exposure is not long enough to elicit a cellular response. It is concluded that these freshly isolated cells are suitable for the study of those effects of hormones and drugs which occur within the first 3 hr after exposure to the ...Continue Reading

References

Nov 1, 1979·Endocrinology·R E Müller, H H Wotiz
Mar 7, 1977·Biochemical and Biophysical Research Communications·B P Sani
Nov 1, 1974·The Journal of Investigative Dermatology·E C GomezP Frost
Mar 1, 1968·Die Naturwissenschaften·R Süss, M Volm
Jul 1, 1964·Proceedings of the Society for Experimental Biology and Medicine·R K FREINKEL

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Citations

Aug 19, 2000·Endocrine Reviews·D Deplewski, R L Rosenfield
Apr 20, 2002·In Vitro Cellular & Developmental Biology. Animal·Robert L RosenfieldNancy Ciletti
Jan 25, 2019·Drug Discoveries & Therapeutics·Indu Lata KanwarVandana Soni
Feb 1, 1992·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·S J LaurentR L Rosenfield
Sep 1, 1991·The Journal of Investigative Dermatology·M I MednieksR L Rosenfield
Nov 1, 1994·The Journal of Investigative Dermatology·K MiyakeR L Rosenfield
Mar 1, 1991·The Journal of Investigative Dermatology·T I DoranE Pacia
Jan 5, 2002·Journal of Agricultural and Food Chemistry·B S JenaK K Sakariah
Oct 23, 2020·Dermatology : International Journal for Clinical and Investigative Dermatology·Christos C ZouboulisFrank C Powell

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