Sec12 binds to Sec16 at transitional ER sites.

PloS One
Elisabeth A MontegnaB S Glick

Abstract

COPII vesicles bud from an ER domain known as the transitional ER (tER). Assembly of the COPII coat is initiated by the transmembrane guanine nucleotide exchange factor Sec12. In the budding yeast Pichia pastoris, Sec12 is concentrated at tER sites. Previously, we found that the tER localization of P. pastoris Sec12 requires a saturable binding partner. We now show that this binding partner is Sec16, a peripheral membrane protein that functions in ER export and tER organization. One line of evidence is that overexpression of Sec12 delocalizes Sec12 to the general ER, but simultaneous overexpression of Sec16 retains overexpressed Sec12 at tER sites. Additionally, when P. pastoris Sec12 is expressed in S. cerevisiae, the exogenous Sec12 localizes to the general ER, but when P. pastoris Sec16 is expressed in the same cells, the exogenous Sec12 is recruited to tER sites. In both of these experimental systems, the ability of Sec16 to recruit Sec12 to tER sites is abolished by deleting a C-terminal fragment of Sec16. Biochemical experiments confirm that this C-terminal fragment of Sec16 binds to the cytosolic domain of Sec12. Similarly, we demonstrate that human Sec12 is concentrated at tER sites, likely due to association with a C-t...Continue Reading

References

Nov 8, 1990·Nature·M J Lewis, H R Pelham
Aug 1, 1984·Molecular and Cellular Biology·M Johnston, R W Davis
Oct 1, 1995·The Journal of Cell Biology·P EspenshadeC A Kaiser
Sep 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·S Nishikawa, A Nakano
Nov 1, 1996·Molecular Biology of the Cell·R E GimenoC A Kaiser
Jul 14, 1997·The Journal of Cell Biology·S I Bannykh, W E Balch
Nov 5, 1997·The Journal of Biological Chemistry·D A ShaywitzC A Kaiser
Oct 3, 2002·Nature Cell Biology·Brooke J BevisBenjamin S Glick
Nov 15, 2002·Proceedings of the National Academy of Sciences of the United States of America·Adam M DeutschbauerRonald W Davis
May 8, 2004·Developmental Cell·Jon SoderholmBenjamin S Glick
Dec 8, 2004·Nature Biotechnology·Stéphanie CabantousGeoffrey S Waldo
Aug 23, 2005·Current Biology : CB·Pamela L ConnerlyBenjamin S Glick
Sep 23, 2006·Nature Methods·Stéphanie Cabantous, Geoffrey S Waldo
Apr 13, 2007·The Journal of Biological Chemistry·Takayuki IinumaKatsuko Tani
Aug 10, 2007·Seminars in Cell & Developmental Biology·Marcus C S Lee, Elizabeth A Miller
Dec 7, 2007·Histochemistry and Cell Biology·Helen Hughes, David J Stephens
Jul 11, 2008·Molecular Biology of the Cell·Viorica IvanCatherine Rabouille
Jul 30, 2009·Journal of Cell Science·Helen HughesDavid J Stephens
Oct 24, 2009·FEBS Letters·Annika Budnik, David J Stephens
Nov 3, 2009·FEBS Letters·Effrosyni Papanikou, Benjamin S Glick
Jan 1, 2010·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Kari PoikonenPekka Saikku
Jan 15, 2010·Traffic·Christopher Russell, Scott M Stagg
Mar 5, 2010·Molecular Biology of the Cell·Polina Shindiapina, Charles Barlowe
Jul 31, 2010·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Konstantin A LukyanovDmitriy M Chudakov
Apr 12, 2011·Nature Cell Biology·Kristen WitteAnjon Audhya

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Citations

Sep 6, 2013·Molecular Biology of the Cell·Nike BharuchaBenjamin S Glick
Feb 5, 2013·Future Microbiology·Brigitte GasserDiethard Mattanovich
Sep 10, 2014·The Journal of Cell Biology·Kota SaitoToshiaki Katada
Oct 1, 2013·Trends in Cell Biology·Rossella VendittiMaria Antonietta De Matteis
Jan 26, 2013·PloS One·Mayuko KoreishiAyano Satoh
Mar 16, 2013·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Foong May Yeong
Nov 19, 2013·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Benjamin S Glick
Jun 18, 2015·Cellular and Molecular Life Sciences : CMLS·Kota Saito, Toshiaki Katada
Jan 27, 2015·Biochemical Society Transactions·Joep Sprangers, Catherine Rabouille
Mar 14, 2013·FEMS Microbiology Reviews·Marizela DelicBrigitte Gasser
Jan 19, 2018·Annual Review of Biophysics·Julien Béthune, Felix T Wieland
Aug 6, 2016·Nature Communications·Ilka WilhelmiFlorian Heyd
Feb 28, 2015·Applied Microbiology and Biotechnology·Verena PuxbaumBrigitte Gasser
Sep 8, 2017·Frontiers in Cell and Developmental Biology·Kota SaitoToshiaki Katada
Jun 23, 2019·The Journal of Cell Biology·Federica G CentonzeHesso Farhan
Nov 8, 2015·The Journal of Biological Chemistry·Michael G HannaAnjon Audhya
Feb 24, 2018·Frontiers in Cell and Developmental Biology·Yasuyuki SudaAkihiko Nakano
Apr 27, 2017·The Journal of Cell Biology·Miharu MaedaKota Saito
Apr 25, 2020·The Journal of Cell Biology·Sudeshna Roy ChowdhuryDibyendu Bhattacharyya
Jun 20, 2018·Histochemistry and Cell Biology·Janine McCaughey, David J Stephens
May 7, 2019·Traffic·Jennifer PeotterAnjon Audhya
Sep 6, 2019·Biochimica Et Biophysica Acta. Molecular Cell Research·Ignacio Bravo-PlazaMiguel A Peñalva
Oct 22, 2019·Trends in Cell Biology·Janine McCaughey, David J Stephens
Nov 17, 2020·Frontiers in Cellular Neuroscience·Chang Geon ChungSung Bae Lee
Dec 21, 2014·Journal of Cell Science·Kerstin D TillmannHesso Farhan
May 18, 2021·Parasitology International·Coh-Ichi Nihei, Masayuki Nakanishi

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Methods Mentioned

BETA
GTPase
nucleotide exchange
immunoprecipitation
co-immunoprecipitation
pull-down
nucleotide
fluorescence microscopy
PCR
dissection
transfection

Software Mentioned

Adobe Photoshop

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