Sec34 is implicated in traffic from the endoplasmic reticulum to the Golgi and exists in a complex with GTC-90 and ldlBp.

The Journal of Biological Chemistry
Eva Loh, Wanjin Hong

Abstract

Sec34p/Grd20p has been implicated in endoplasmic reticulum (ER)-to-Golgi transport and/or post-Golgi trafficking events and exists in a protein complex consisting of at least eight subunits in yeast. Although the mammalian counterpart (Sec34) of Sec34p has been molecularly identified, its role and interacting partners remain undefined. In this study, we have prepared antibodies specifically against the recombinant N-terminal fragment of Sec34 that recognize a polypeptide of about 93 kDa and label the Golgi apparatus. In a well-characterized semi-intact cell assay that reconstitutes transport of the envelope glycoprotein (VSVG) of vesicular stomatitis virus from the ER to the Golgi apparatus, anti-Sec34 antibodies inhibited the transport in a dose-dependent manner. The inhibition by anti-Sec34 antibodies could be neutralized by a noninhibitory amount of the antigen. Large-scale immunoprecipitation of rat liver cytosol with immobilized anti-Sec34 antibodies has co-immunoprecipitated GTC-90 and ldlBp, two peripheral Golgi proteins previously shown to exist in separate protein complexes. Two mammalian homologues (Dor1 and Cod1) of the yeast Sec34 complex were similarly recovered in the Sec34 immunoprecipitates. When expressed in tr...Continue Reading

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Citations

Jul 17, 2010·BMC Evolutionary Biology·Rita QuentalAntónio Amorim
Nov 6, 2004·Journal of Neuroinflammation·Lars Rönnbäck, Elisabeth Hansson
Feb 25, 2005·The Journal of Cell Biology·Sergey N Zolov, Vladimir V Lupashin
Feb 4, 2012·Traffic·Victoria J Miller, Daniel Ungar
Jul 22, 2006·Experimental Cell Research·Eliza VasileMonty Krieger
Apr 27, 2004·Nature Medicine·Xiaohua WuHudson H Freeze
Jul 28, 2019·Metallomics : Integrated Biometal Science·Xiuxiu WangJing Zhao
Mar 30, 2004·The Journal of Biological Chemistry·Eva Loh, Wanjin Hong

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