PMID: 946141Jan 1, 1976

Second antibody chemically linked to cellulose for the separation of bound and free hormone: an improvement over soluble second antibody in gonadotrophin radioimmunoassay

Acta Endocrinologica
P R KoninckxP De Moor

Abstract

Coupling the second antibody to a solid pase (DASP) was found to be a definite improvement over the classical technique of soluble second antibody (DA) separation of bound and free hormone in the radioimmunoassays of gonadotrophins in plasma or in serum. The duration (2.5 to 7.5 min) and the pH (10.5 to 12.5) of the cyanogen bromide activation of microcristalline cellulose did not affect the coupling capacity or the immunoreactivity of the second antibody, nor did it augment aspecific adsorption of free gonadotrophin on the cellulose matrix. Precision of duplicates was comparable for both methods but the accuracy and the detection limit of the solid phase system were higher due to a better separation of bound and free hormone, to the lower blank value and to the absence of aspecific interference of protein concentration. Cost was lower since much less second antibody was needed in the solid phase system than in the soluble system and since the experimental procedures were simplified by the absence of prozoning effect and the solid nature of the cellulose matrix.

Citations

Aug 1, 1987·Calcified Tissue International·J R M'Buyamba-KabanguA Amery
Aug 1, 1991·Calcified Tissue International·D VanderschuerenR Bouillon
Jan 1, 1983·Journal of Immunological Methods·P Englebienne, G Slegers
Jan 1, 1976·European Journal of Obstetrics, Gynecology, and Reproductive Biology·I A BrosensR De Hertogh
Jan 1, 1976·European Journal of Obstetrics, Gynecology, and Reproductive Biology·P Koninckx, P De Moor
Jun 1, 1981·British Journal of Obstetrics and Gynaecology·P R KoninckxF A Van Assche
Jan 1, 1982·Endocrine Research Communications·W E Findley, A Steinberger

Related Concepts

Follicle Stimulating Hormone
Goat
Immunologic Memory
Luteozyman
Radioimmunoassay

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