Second generation snake antivenomics: comparing immunoaffinity and immunodepletion protocols

Toxicon : Official Journal of the International Society on Toxinology
Davinia PlaJuan J Calvete

Abstract

A second generation antivenomics protocol, based on affinity chromatography, was compared with a previously (first generation) immunodepletion protocol using as a proof of principle the pan-African EchiTAb-Plus-ICP(®) IgG antivenom and the venoms of Echis ocellatus, Bitis arietans, and African spitting cobras. The antivenom showed qualitatively similar immunoreactivity patterns using either antivenomic approach. Quantitative departures were noticed between both methods, which may be ascribed to differences in calculating the relative amounts of the non-recognized venom proteins. The smoother baseline in chromatograms of the affinity column allowed better resolution and more accurate quantification of the antivenomic outcome than the original immunodepletion protocol. Our results indicate that both methods can be used interchangeably to investigate the in vitro immunoreactivity of antivenoms. However, advantages of the second generation antivenomics are the possibility of analyzing F(ab')(2) antivenoms and the reusability of the affinity columns. These features contribute to the generalization, economy and reproducibility of the method.

References

Jul 10, 1999·Toxicon : Official Journal of the International Society on Toxinology·B J Hawgood
Jun 17, 2003·Journal of Toxicology. Clinical Toxicology·David G Lalloo, R David G Theakston
Feb 3, 2005·Toxicon : Official Journal of the International Society on Toxinology·Sean M Munekiyo, Stephen P Mackessy
Apr 20, 2005·Transactions of the Royal Society of Tropical Medicine and Hygiene·J M GutiérrezG Rojas
Jun 2, 2006·PLoS Medicine·Simon C WagstaffRobert A Harrison
Nov 22, 2007·Biochemical and Biophysical Research Communications·Simon C WagstaffRobert A Harrison
Mar 24, 2009·FEBS Letters·Juan J CalveteJosé María Gutiérrez
Oct 31, 2009·Toxicon : Official Journal of the International Society on Toxinology·S B AbubakarUNKNOWN Nigeria-UK EchiTab Study Group
Dec 23, 2009·PLoS Neglected Tropical Diseases·Robert A HarrisonDavid G Lalloo
Dec 29, 2009·Toxicon : Official Journal of the International Society on Toxinology·Juan J Calvete
Jan 30, 2010·Lancet·David WilliamsUNKNOWN International Society on Toxinology
Jun 4, 2010·The American Journal of Tropical Medicine and Hygiene·Juan J CalveteJosé María Gutiérrez
Nov 5, 2010·PLoS Neglected Tropical Diseases·Nicholas R CasewellRobert A Harrison
Mar 25, 2011·Biologicals : Journal of the International Association of Biological Standardization·José María GutiérrezThierry Burnouf
Jul 5, 2011·Journal of Proteomics·Robert A HarrisonSimon C Wagstaff
Jul 13, 2011·Inflammation & Allergy Drug Targets·José María GutiérrezYamileth Angulo
Nov 18, 2011·Expert Review of Proteomics·Juan J Calvete

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Citations

Aug 7, 2014·Transactions of the Royal Society of Tropical Medicine and Hygiene·José María Gutiérrez
Sep 27, 2013·PLoS Neglected Tropical Diseases·Leijiane F SousaAna M Moura-da-Silva
Dec 18, 2014·Toxins·Juan J CalveteJosé María Gutiérrez
Dec 1, 2015·Toxicon : Official Journal of the International Society on Toxinology·José María Gutiérrez
Jul 15, 2015·Toxicon : Official Journal of the International Society on Toxinology·Maitreyee SharmaRobin Doley
Jun 26, 2012·Toxicon : Official Journal of the International Society on Toxinology·Juan J Calvete
Dec 3, 2014·Biologicals : Journal of the International Association of Biological Standardization·Mariángela VargasGuillermo León
Jun 24, 2015·Toxicon : Official Journal of the International Society on Toxinology·Cynthia ArroyoGuillermo León
Apr 13, 2013·Toxicon : Official Journal of the International Society on Toxinology·Juan J Calvete
Dec 3, 2014·Toxicon : Official Journal of the International Society on Toxinology·Rajeev Kungur BrahmaRobin Doley
Jun 4, 2016·Toxicon : Official Journal of the International Society on Toxinology·Kitisak SintiprungratKavi Ratanabanangkoon
Jul 6, 2016·Toxicon : Official Journal of the International Society on Toxinology·Juan J CalveteJosé María Gutiérrez
Oct 28, 2016·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Rita de Cássia O CollaçoLéa Rodrigues-Simioni
Aug 27, 2014·The American Journal of Tropical Medicine and Hygiene·María HerreraJosé María Gutiérrez
Aug 5, 2018·Rapid Communications in Mass Spectrometry : RCM·Parviz GhezellouBernhard Spengler
Sep 29, 2018·Toxins·Line LedsgaardAndreas Hougaard Laustsen
Feb 29, 2020·Annual Review of Analytical Chemistry·Anthony J SaviolaJohn R Yates
Jun 28, 2020·Molecular Ecology·H Lisle GibbsJuan J Calvete
May 17, 2017·The Journal of Venomous Animals and Toxins Including Tropical Diseases·Ricardo Teixeira-AraújoCarlos Correa-Netto
Dec 12, 2018·The Journal of Venomous Animals and Toxins Including Tropical Diseases·Isadora Sousa de OliveiraEliane Candiani Arantes
Apr 20, 2017·Toxins·Anjana SilvaGeoffrey K Isbister

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