Second-harmonic imaging of cornea after intrastromal femtosecond laser ablation

Journal of Biomedical Optics
Meng HanJosef F Bille

Abstract

Nonlinear laser scanning microscopy is widely used for noninvasive imaging in cell biology and tissue physiology. However, multiphoton fluorescence imaging of dense, transparent connective tissue (e.g., cornea) is challenging since sophisticated labeling or slicing is necessary. High-resolution, high-contrast second harmonic generation (SHG) imaging of corneal tissue based on the intrinsic structure of collagen is discussed. The three-dimensional corneal ultrastructure in depths up to hundreds of microns can be probed noninvasively, without any staining or mechanical slicing. As an important application of second harmonic imaging in ophthalmology, the modification of corneal ultrastructure using femtosecond laser intrastromal ablation is systematically investigated to evaluate next-generation refractive surgical approaches.

References

Apr 6, 1990·Science·W DenkW W Webb
Dec 10, 1999·Biophysical Journal·P J CampagnolaL M Loew
Mar 29, 2000·Biophysical Journal·G H Patterson, D W Piston
Feb 27, 2001·Biophysical Journal·L MoreauxJ Mertz
May 4, 2001·Micron : the International Research and Review Journal for Microscopy·R GauderonC J Sheppard
May 4, 2001·Micron : the International Research and Review Journal for Microscopy·R GauderonC J Sheppard
Aug 23, 2001·Journal of Biomedical Optics·P J CampagnolaL M Loew
May 23, 2002·Biophysical Journal·Patrick StollerAlexander M Rubenchik
Jan 14, 2003·Biophysical Journal·Giuseppe ChiricoAlberto Diaspro
Feb 11, 2003·Journal of Structural Biology·Guy CoxMark D Gorrell

❮ Previous
Next ❯

Citations

Aug 7, 2007·Biochemistry·Yoshikazu ImanishiYiannis Koutalos
Feb 28, 2007·Investigative Ophthalmology & Visual Science·Naoyuki MorishigeJames V Jester
Mar 11, 2006·Journal of Biomedical Optics·Meng HanJosef F Bille
Mar 11, 2006·Journal of Biomedical Optics·Julia G LyubovitskyBruce J Tromberg
Sep 4, 2009·Journal of Biomedical Optics·Szu-Yu ChenChi-Kuang Sun
Nov 10, 2009·Journal of Biomedical Optics·Wei-Liang ChenChen-Yuan Dong
Nov 4, 2006·Journal of Cataract and Refractive Surgery·Naoyuki MorishigeJames V Jester
Nov 20, 2008·Journal of Microscopy·B Vohnsen, P Artal
Sep 30, 2016·Advances in Wound Care·Cynthia Yu-Wai-Man, Peng Tee Khaw
Mar 31, 2015·Experimental Eye Research·Andrew J QuantockJames V Jester
Nov 1, 2005·Biophysical Journal·Sergey V PlotnikovWilliam A Mohler
Dec 19, 2006·Biophysical Journal·Christopher B RaubSteven C George
Jan 1, 2013·Journal of Functional Biomaterials·Samantha L WilsonAndrew Hopkinson
Sep 19, 2018·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Yashar Seyed-RazaviPedram Hamrah
Sep 22, 2007·Lasers in Surgery and Medicine·Hui SunJosef F Bille
Jan 26, 2011·Optics Express·L HoffartI Brunette
Feb 7, 2015·Biomedical Optics Express·Luke J MortensenCharles P Lin
Apr 10, 2008·Current Eye Research·Matthias HildKarsten König

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Imaging in CNS

Here is the latest research on cell imaging and imaging modalities, including light-sheet microscopy, in the central nervous system.