Second-site reversion of a dysfunctional mutation in a conserved region of the tobacco mosaic tobamovirus movement protein

Virology
C M Deom, X Z He

Abstract

The N-terminal two-thirds of tobamovirus movement proteins (MPs) contain two well conserved regions. Within region I (amino acids 56-96) is an area predicted by computer analysis to have loop secondary structure (amino acids 76-87). A single or two double amino acid mutations were introduced into the loop in region I of the TMV MP to destabilize the structure. The three mutant MPs were defective in movement function. The single amino acid mutation resulted in a Pro81-->Ser substitution. The mutant virus, TP81S, containing the Pro81-->Ser substitution, was propagated on a transgenic line of Nicotiana tabacum that expresses the sunn-hemp mosaic tobamovirus MP. Inoculation of virus progeny from the transgenic plants onto hypersensitive N. tabacum indicated the presence of infectious virus at a low frequency. Necrotic lesions were detected at 4 days postinoculation, 2 days later than those induced by wild-type TMV. Inoculation of virus extracted from necrotic lesions onto N. tabacum resulted in a delayed and attenuated systemic infection relative to that induced by TMV, indicating that a second-site mutation restored movement function rather than a reversion of the original mutation. Sequence analysis revealed that the revertant MP...Continue Reading

References

Apr 17, 1992·Cell·C M DeomR N Beachy
May 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·C M DeomR N Beachy
Sep 1, 1995·Molecular Plant-microbe Interactions : MPMI·C A FenczikR N Beachy
Oct 1, 1996·The Plant Cell·J. C. CarringtonM. C. Schaad

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Citations

Jun 6, 2002·The Plant Journal : for Cell and Molecular Biology·Rachel L TothSean Chapman
Apr 5, 2008·PLoS Pathogens·Hannes VoglerManfred Heinlein
Dec 25, 2015·Virology·Krin S MannRalf G Dietzgen
Jan 10, 2008·The Plant Journal : for Cell and Molecular Biology·Carine CharronCarole Caranta

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