PMID: 11929267Apr 4, 2002Paper

Second-sphere contributions to substrate-analogue binding in iron(III) superoxide dismutase

Journal of the American Chemical Society
Juan XieThomas C Brunold

Abstract

A combination of spectroscopic and computational methods has been employed to explore the nature of the yellow and pink low-temperature azide adducts of iron(III) superoxide dismutase (N(3)-FeSOD), which have been known for more than two decades. Variable-temperature variable-field magnetic circular dichroism (MCD) data suggest that both species possess similar ferric centers with a single azide ligand bound, contradicting previous proposals invoking two azide ligands in the pink form. Complementary data obtained on the azide complex of the Q69E FeSOD mutant reveal that relatively minor perturbations in the metal-center environment are sufficient to produce significant spectral changes; the Q69E N(3)-FeSOD species is red in color at all temperatures. Resonance Raman (RR) spectra of the wild-type and Q69E mutant N(3)-FeSOD complexes are consistent with similar Fe-N(3) units in all three species; however, variations in energies and relative intensities of the RR features associated with this unit reveal subtle differences in (N(3)(-))-Fe(3+) bonding. To understand these differences on a quantitative level, density functional theory and semiempirical INDO/S-CI calculations have been performed on N(3)-FeSOD models. These computatio...Continue Reading

References

Jan 1, 1991·Free Radical Research Communications·W C StallingsM L Ludwig
Apr 4, 2002·Journal of the American Chemical Society·Emine YikilmazAnne-Frances Miller

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Citations

Mar 25, 2010·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Olivia E JohnsonThomas C Brunold
Apr 6, 2004·Current Opinion in Chemical Biology·Anne-Frances Miller
Aug 8, 2008·Chemical Communications : Chem Comm·Luciano CuestaJonathan L Sessler
Mar 26, 2008·The Journal of Chemical Physics·Dmitry Ganyushin, Frank Neese
Jun 28, 2006·Journal of Computational Chemistry·Lubomír RulísekUlf Ryde
Oct 16, 2002·The Journal of Biological Chemistry·William B Greenleaf, David N Silverman
Sep 14, 2011·Chemistry, an Asian Journal·Sandip A PujariKrishna P Kaliappan
Aug 10, 2006·Chemical Reviews·Stephen W Ragsdale
Aug 4, 2018·Journal of the American Chemical Society·Eric W DahlNathaniel K Szymczak
Nov 26, 2009·Biochimica Et Biophysica Acta·Anne-Frances MillerSurekha Vathyam

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