PMID: 9193784May 1, 1997Paper

Secondary alcohol dehydrogenase as a marker of the conversion between progestagens and androgens in the rat testis

Cellular and Molecular Biology
J A de OliveiraA A Rosa e Silva

Abstract

Supraphysiological doses of LHRH-Analogue blocked the C21 to C19 steroid conversion in the mature Wistar rats testis. It was associated with inhibition of the NAD-dependent secondary alcohol-dehydrogenase (A-D II) histochemical reaction in the Leydig cells. Under this condition the treated group exhibited lower testis, seminal vesicle and prostate weights, intratesticular (IT) and plasmatic (PL) increased progesterone (P4) and decreased testosterone (T) concentrations. We also observed a decrease in the IT androstenedione (delta 4) concentration without pregnenolone (P5) change. All these data confirm a chemical castration pointing to a blockade at the level of the P450C21scc (17 alpha-hydroxylase/17-20 desmolase) enzyme complex. After hCG administration there is no difference in sexual gland weights, while steroid's biosynthesis are stimulated and all IT and PL steroid concentrations increase. A-D II showed a lower optical density in the LHRH-A treated groups and no differences in the hCG rats. The hydroxylase or lyase activity of the P450C21scc may change under certain hormonal conditions as occurs in adrenarche, probably due to conformational changes in the active site of the enzyme system since it is encoded by only one gen...Continue Reading

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