Secondary-ion mass spectrometry of genetically encoded targets

Angewandte Chemie
Ingrid C VrejaSilvio O Rizzoli

Abstract

Secondary ion mass spectrometry (SIMS) is generally used in imaging the isotopic composition of various materials. It is becoming increasingly popular in biology, especially for investigations of cellular metabolism. However, individual proteins are difficult to identify in SIMS, which limits the ability of this technology to study individual compartments or protein complexes. We present a method for specific protein isotopic and fluorescence labeling (SPILL), based on a novel click reaction with isotopic probes. Using this method, we added (19) F-enriched labels to different proteins, and visualized them by NanoSIMS and fluorescence microscopy. The (19) F signal allowed the precise visualization of the protein of interest, with minimal background, and enabled correlative studies of protein distribution and cellular metabolism or composition. SPILL can be applied to biological systems suitable for click chemistry, which include most cell-culture systems, as well as small model organisms.

References

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Citations

Jun 20, 2016·Current Opinion in Structural Biology·Petra Neumann-Staubitz, Heinz Neumann
Aug 11, 2016·Current Opinion in Biotechnology·Haibo JiangNiculina Musat
Aug 27, 2015·Molecular & Cellular Proteomics : MCP·Daniela C Dieterich, Michael R Kreutz
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Dec 9, 2017·Angewandte Chemie·Linwen Zhang, Akos Vertes
May 4, 2017·Neurophotonics·Katharina N RichterJelena Lovric
Jan 8, 2019·Angewandte Chemie·Selda KabatasNhu T N Phan
Aug 15, 2020·Frontiers in Behavioral Neuroscience·Elisa A Bonnin, Silvio O Rizzoli
Oct 13, 2016·The Biochemical Journal·Manuel MaidornFelipe Opazo
Sep 29, 2017·Biointerphases·Jamie NuñezChristopher R Anderton
Sep 29, 2019·Biotechnology Journal·Wenyi ChenJingjing Wan
Sep 29, 2019·Nature Communications·Hansjörg GötzkeSteffen Frey
Dec 20, 2019·Analytical Chemistry·Brittney L Gorman, Mary L Kraft
Jan 13, 2021·BMC Molecular and Cell Biology·Elisa A BonninSilvio O Rizzoli
Mar 18, 2021·Cell Reports·Sebastian JähneViola Priesemann
Jun 9, 2020·ACS Nano·Brittney L GormanMary L Kraft
Jan 29, 2020·Trends in Cell Biology·Johan DecelleNiculina Musat
Aug 1, 2021·Nature Communications·Xavier Rovira-ClavéGarry P Nolan
Dec 5, 2017·Biochemistry·Alf Honigmann, André Nadler

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Methods Mentioned

BETA
fluorescence imaging
Fluorescence
fluorescence microscopy

Software Mentioned

SPILL

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