Secondary isotope effects in liquid chromatography behaviour of 2H and 3H labelled solutes and solvents

Journal of Chromatography. a
Alain ValleixAlain Tchapla

Abstract

The separation of solutes that differ only in the extent of isotopic substitution of their hydrogen atoms, using either mixtures of isotopically non-modified or perdeuterated solvents as mobile phases, is described. The occurrence of a secondary isotope effect is demonstrated in reversed-phase liquid chromatography, which is independent of the nature of the stationary phase (different octadecyl-bonded silicas, an embedded alkylamide-bonded silica, as well as one polymeric stationary phase were tested), and the water content and the nature of organic modifier of the mobile phase. The separation of 24 structurally different isotopologue pairs (apolar compounds and polar compounds with exchangeable or non-exchangeable hydrogen atoms) is examined using reversed-phase liquid chromatography. It is found that the greater the number of isotopically substituted hydrogen atoms in a given organic solute, the better is the separation of a particular isotopologue pair. The single secondary isotope effect is shown to be dependent on the number of isotopic substitutions. The greater the number of these substitutions, the smaller is the single isotope effect. The single secondary isotope effect is higher for aromatic hydrocarbons than for alip...Continue Reading

Citations

Jun 27, 2014·Journal of Pharmaceutical and Biomedical Analysis·Zoltán BéniCsaba Szántay
Aug 18, 2017·Angewandte Chemie·Jens AtzrodtMarc Reid
Aug 6, 2011·Physical Chemistry Chemical Physics : PCCP·Masami ShibukawaShingo Saito
Feb 24, 2017·Journal of Molecular Modeling·Félix MoncadaAndrés Reyes
Oct 11, 2013·Proteomes·Barbara DeracinoisYannis Karamanos
May 13, 2021·Analytica Chimica Acta·Nimisha ThakurDaniel W Armstrong
Jan 15, 2019·Journal of Proteome Research·Jaehun JungJong-Seo Kim
Dec 21, 2007·Journal of the American Chemical Society·Eliot P BotosoaGérald S Remaud

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