Secondary metabolites of the grapevine pathogen Eutypa lata inhibit mitochondrial respiration, based on a model bioassay using the yeast Saccharomyces cerevisiae

Current Microbiology
Jong H KimBruce C Campbell

Abstract

Acetylenic phenols and a chromene isolated from the grapevine fungal pathogen Eutypa lata were examined for mode of toxicity. The compounds included eutypine (4-hydroxy-3-[3-methyl-3-butene-1-ynyl] benzyl aldehyde), eutypinol (4-hydroxy-3-[3-methyl-3-butene-1-ynyl] benzyl alcohol), eulatachromene, 2- isoprenyl-5-formyl-benzofuran, siccayne, and eulatinol. A bioassay using the yeast Saccharomyces cerevisiae showed that all compounds were either lethal or inhibited growth. A respiratory assay using 2,3,5-triphenyltetrazolium (TTC) indicated that eutypinol and eulatachromene inhibited mitochondrial respiration in wild-type yeast. Bioassays also showed that 2- isoprenyl-5-formyl-benzofuran and siccayne inhibited mitochondrial respiration in the S. cerevisiae deletion mutant vph2Delta, lacking a vacuolar type H (+) ATPase (V-ATPase) assembly protein. Cell growth of tsa1Delta, a deletion mutant of S. cerevisiae lacking a thioredoxin peroxidase (cTPx I), was greatly reduced when grown on media containing eutypinol or eulatachromene and exposed to hydrogen peroxide (H(2)O(2)) as an oxidative stress. This reduction in growth establishes the toxic mode of action of these compounds through inhibition of mitochondrial respiration.

Citations

Nov 2, 2013·Journal of Natural Products·Benjarong ThongbaiMarc Stadler
Nov 14, 2013·Chemistry and Physics of Lipids·David J MontefuscoYusuf A Hannun
Jul 23, 2013·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Dmitry V KuklevValery M Dembitsky
Sep 15, 2009·Comptes rendus biologies·Philippe LarignonChristophe Bertsch
Apr 5, 2008·Progress in Lipid Research·Robert E Minto, Brenda J Blacklock
Feb 27, 2007·Mitochondrion·David M Rhoads, Chalivendra C Subbaiah
Feb 3, 2006·Natural Product Reports·Daniel Jiménez-TejaIsidro González Collado
Mar 15, 2005·Yeast
Jul 26, 2019·The Journal of Antibiotics·Kazuki KannoKouji Kuramochi
Feb 2, 2012·Toxins·Anna AndolfiAntonio Evidente
Jun 29, 2018·Scientific Reports·Victor González-MenéndezOlga Genilloud
Jul 1, 2005·Functional Plant Biology : FPB·Jérome RudellePierrette Fleurat-Lessard
Sep 3, 2014·Journal of Natural Products·Guillermo A Guerrero-VásquezFrancisco A Macías

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