Secondary solute transport in bacteria
No abstract listed.
pH-dependent changes in proton:substrate stoichiometries during active transport in Escherichia coli membrane vesicles
Mechanism of lactose translocation in membrane vesicles from Escherichia coli. 1. Effect of pH on efflux, exchange, and counterflow
Mechanism of lactose translocation in membrane vesicles from Escherichia coli. 2. Effect of imposed delata psi, delta pH, and Delta mu H+
The N-terminal 22 amino acid residues in the lactose permease of Escherichia coli are not obligatory for membrane insertion or transport activity
Excretion of putrescine by the putrescine-ornithine antiporter encoded by the potE gene of Escherichia coli
Mechanics of solute translocation catalyzed by enzyme IImtl of the phosphoenolpyruvate-dependent phosphotransferase system of Escherichia coli
Proposed partial beta-structures for lac permease and the Na+/H+ antiporter which use similar transport and H+ coupling mechanisms
Efflux-mediated multidrug resistance in Bacillus subtilis: similarities and dissimilarities with the mammalian system
Organization and stability of a polytopic membrane protein: deletion analysis of the lactose permease of Escherichia coli
Sequential truncation of the lactose permease over a three-amino acid sequence near the carboxyl terminus leads to progressive loss of activity and stability
Subunit structure and activity of the mannitol-specific enzyme II of the Escherichia coli phosphoenolpyruvate-dependent phosphotransferase system solubilized in detergent
Information content of amino acid residues in putative helix VIII of the lac permease from Escherichia coli
Characterization of site-directed mutants in the lac permease of Escherichia coli. 2. Glutamate-325 replacements
Effect of distance and orientation between arginine-302, histidine-322, and glutamate-325 on the activity of lac permease from Escherichia coli
In vitro membrane assembly of a polytopic, transmembrane protein results in an enzymatically active conformation
A five-residue sequence near the carboxyl terminus of the polytopic membrane protein lac permease is required for stability within the membrane
Regulation of Escherichia coli adenylate cyclase activity by the phosphoenolpyruvate:sugar phosphotransferase system
Bioenergetic coupling to protonmotive force: should we be considering hydronium ion coordination and not group protonation?
lac permease of Escherichia coli: histidine-322 and glutamic acid-325 may be components of a charge-relay system
Variable stoichiometry of phosphate-linked anion exchange in Streptococcus lactis: implications for the mechanism of sugar phosphate transport by bacteria
Role of an electrical potential in the coupling of metabolic energy to active transport by membrane vesicles of Escherichia coli
Mechanism of glutamate transport in Escherichia coli B. 1. Proton-dependent and sodium ion dependent binding of glutamate to a glutamate carrier in the cytoplasmic membrane
Mechanism of lactose translocation in proteoliposomes reconstituted with lac carrier protein purified from Escherichia coli. 2. Deuterium solvent isotope effects
Reconstitution of active transport in proteoliposomes containing cytochrome o oxidase and lac carrier protein purified from Escherichia coli
Relationships between the Na+-H+ antiport activity and the components of the electrochemical proton gradient in Escherichia coli membrane vesicles
Kinetic and steady-state investigations of solute accumulation in bacterial membranes by continuously monitoring the radioactivity in the effluent of flow-dialysis experiments
Glucose 6-phosphate transport in membrane vesicles isolated from Escherichia coli: effect of imposed electrical potential and pH gradient
Regulation of lactose permease activity by the phosphoenolpyruvate:sugar phosphotransferase system: evidence for direct binding of the glucose-specific enzyme III to the lactose permease
Exchange, efflux, and substrate binding by cysteine mutants of the lactose permease of Escherichia coli
Kinetic properties of Na(+) -H(+) antiport in Escherichia coli membrane vesicles: Effects of imposed electrical potential, proton gradient, and internal pH
Fluorescence of native single-Trp mutants in the lactose permease from Escherichia coli: structural properties and evidence for a substrate-induced conformational change
Chemiosmotic concept of the membrane bioenergetics: what is already clear and what is still waiting for elucidation?
A suppressor analysis of residues involved in cation transport in the lactose permease: identification of a coupling sensor
Non-physiological expression of UhpT does not lead to uncontrolled leakage of sugar phosphates out of Escherichia coli cells
Only one of the charged amino acids located in membrane-spanning regions is important for the function of the Saccharomyces cerevisiae uracil permease
Inhibition of Escherichia coli respiratory enzymes by the lactoperoxidase-hydrogen peroxide-thiocyanate antimicrobial system
Engineering a terbium-binding site into an integral membrane protein for luminescence energy transfer
Surface-exposed positions in the transmembrane helices of the lactose permease of Escherichia coli determined by intermolecular thiol cross-linking
Regulation of bacterial sugar-H+ symport by phosphoenolpyruvate-dependent enzyme I/HPr-mediated phosphorylation
Site-directed spin labeling and chemical crosslinking demonstrate that helix V is close to helices VII and VIII in the lactose permease of Escherichia coli
The membrane proteins SiaQ and SiaM form an essential stoichiometric complex in the sialic acid tripartite ATP-independent periplasmic (TRAP) transporter SiaPQM (VC1777-1779) from Vibrio cholerae.
Transport of citrate catalyzed by the sodium-dependent citrate carrier of Klebsiella pneumoniae is obligatorily coupled to the transport of two sodium ions
Amplified expression, purification and functional reconstitution of the dipeptide and tripeptide transport protein of Lactococcus lactis
An alternative succinate (2-oxoglutarate) transport system in Rhizobium tropici is induced in nodules of Phaseolus vulgaris
Functional reconstitution of SdcS, a Na+-coupled dicarboxylate carrier protein from Staphylococcus aureus
Pyridoxal 5-phosphate inhibition of substrate selectivity mutants of UhpT, the sugar 6-phosphate carrier of Escherichia coli
Conserved low-affinity nickel-binding amino acids are essential for the function of the nickel permease NixA of Helicobacter pylori
Altered substrate selection of the melibiose transporter (MelY) of Enterobacter cloacae involving point mutations in Leu-88, Leu-91, and Ala-182 that confer enhanced maltose transport
Adaptation to life at micromolar nutrient levels: the regulation of Escherichia coli glucose transport by endoinduction and cAMP
Multidrug efflux pumps from Enterobacteriaceae, Vibrio cholerae and Staphylococcus aureus bacterial food pathogens
A gate-free pathway for substrate release from the inward-facing state of the Na⁺-galactose transporter
The substrate-binding protein imposes directionality on an electrochemical sodium gradient-driven TRAP transporter
Alteration of Na(+)-coupled transport in site-directed mutants of the melibiose carrier of Escherichia coli
Evaluation of the tannic acid inhibitory effect against the NorA efflux pump of Staphylococcus aureus
Characterization of the l-alanine exporter AlaE of Escherichia coli and its potential role in protecting cells from a toxic-level accumulation of l-alanine and its derivatives
Energetics of wild-type and mutant multidrug resistance secondary transporter LmrP of Lactococcus lactis
Sugar recognition mutants of the melibiose carrier of Escherichia coli: possible structural information concerning the arrangement of membrane-bound helices and sugar/cation recognition site
Secondary structure components and properties of the melibiose permease from Escherichia coli: a fourier transform infrared spectroscopy analysis
Characterization of a novel sialic acid transporter of the sodium solute symporter (SSS) family and in vivo comparison with known bacterial sialic acid transporters
The role of local hydration and hydrogen-bonding dynamics in ion and solute release from ion-coupled secondary transporters
Syntheses and properties of photoactivatable sugar derivatives designed to probe the sugar-binding site of melibiose permease
Proximity relationships between helices I and XI or XII in the lactose permease of Escherichia coli determined by site-directed thiol cross-linking
Engineering a metal binding site within a polytopic membrane protein, the lactose permease of Escherichia coli
Unidirectional reconstitution into detergent-destabilized liposomes of the purified lactose transport system of Streptococcus thermophilus.
SecDF of Bacillus subtilis, a molecular Siamese twin required for the efficient secretion of proteins.
Phosphatidylethanolamine is required for in vivo function of the membrane-associated lactose permease of Escherichia coli.
Chaperonin-promoted post-translational membrane insertion of a multispanning membrane protein lactose permease.
Membrane topology of the sodium ion-dependent citrate carrier of Klebsiella pneumoniae. Evidence for a new structural class of secondary transporters.
Glycine betaine fluxes in Lactobacillus plantarum during osmostasis and hyper- and hypo-osmotic shock.
Transmembrane segment 11 of UhpT, the sugar phosphate carrier of Escherichia coli, is an alpha-helix that carries determinants of substrate selectivity
Locking Two Rigid-body Bundles in an Outward-Facing Conformation: The Ion-coupling Mechanism in a LeuT-fold Transporter
Effect of energy deprivation on metabolite release by anaerobic marine naphthalene-degrading sulfate-reducing bacteria.
Structural studies of the melibiose permease of Escherichia coli by fluorescence resonance energy transfer. I. Evidence for ion-induced conformational change.
Study of amide-proton exchange of Escherichia coli melibiose permease by attenuated total reflection-Fourier transform infrared spectroscopy: evidence of structure modulation by substrate binding
Kinetic analysis of lactose and proton coupling in Glu379 mutants of the lactose transport protein of Streptococcus thermophilus.
Uncoupling in secondary transport proteins. A mechanistic explanation for mutants of lac permease with an uncoupled phenotype.
Thermodynamic secrets of multidrug resistance: A new take on transport mechanisms of secondary active antiporters
Evaluation of secondary structure of OxlT, the oxalate transporter of Oxalobacter formigenes, by circular dichroism spectroscopy.
Identification of an Na+-dependent malonate transporter of Malonomonas rubra and its dependence on two separate genes
Arg-52 in the melibiose carrier of Escherichia coli is important for cation-coupled sugar transport and participates in an intrahelical salt bridge
Molecular cloning and functional expression in lactobacillus plantarum 80 of xylT, encoding the D-xylose-H+ symporter of Lactobacillus brevis
Helix proximity and ligand-induced conformational changes in the lactose permease of Escherichia coli determined by site-directed chemical crosslinking
Helices VII and X in the lactose permease of Escherichia coli: proximity and ligand-induced distance changes
Transmembrane helix tilting and ligand-induced conformational changes in the lactose permease determined by site-directed chemical crosslinking in situ
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