PMID: 53210Dec 15, 1975

Secondary specific immune response in vitro to MSV tumor cells

International Journal of Cancer. Journal International Du Cancer
A SenikJ P Levy


The interactions which occur between antigenic tumor cells and normal or immune lymphoid cells in a 3-day in vitro culture, have been studied with a murine sarcoma virus (MSV)-induced tumor. The 3H-thymidine incorporation of lymphoma cells growing in suspension, and the radioactive-chromium release of freshly sampled lymphoma cells regularly added to the culture, have been compared to determine the part played by immune lymphoid cells in cytolysis and cytostasis of the tumor-cell population. The cytolytic activity increases in the culture from day 0 to day 3. It is due, predominantly, to T-cells, and remains specific to antigens shared by MSV tumors and related lymphomas. This activity would be difficult to detect unless freshly sampled ascitic cells were used as targets, since the lymphoma cells spontaneously lose a part of their sensitivity to immune cytolysis during in vitro culture. The method used in the present experiments is a secondary chromium release test (SCRT), which measures the invitro secondary stimulation of cytotoxic T-lymphocytes (CTL) by tumor cells. In the absence of stimulatory cells, the CTL activity would have rapidly fallen in vitro. The cytostatic activity also increases during the 3 days in vitro, in p...Continue Reading


Dec 15, 1975·International Journal of Cancer. Journal International Du Cancer·A SenikJ P Levy
Sep 15, 1974·International Journal of Cancer. Journal International Du Cancer·A SenikJ P Levy
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Related Concepts

Antigenic Specificity
Tumor Antigens
Lymphocyte Immune Globulin, Anti-Thymocyte Globulin (Equine)
Cell Adhesion
Hemolytic Complement
Microcytotoxicity Test
Iron Chelating Agents
Lymphoid Cells

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