Secondary structure and lipid contact of a peptide antibiotic in phospholipid bilayers by REDOR

Biophysical Journal
Orsolya TokeJacob Schaefer

Abstract

The chemical shifts of specific (13)C and (15)N labels distributed throughout KIAGKIA-KIAGKIA-KIAGKIA (K3), an amphiphilic 21-residue antimicrobial peptide, prove that the peptide is in an all alpha-helical conformation in the bilayers of multilamellar vesicles (MLVs) containing dipalmitoylphosphatidylcholine and dipalmitoylphosphatidylglycerol (1:1). Rotational-echo double-resonance (REDOR) (13)C[(31)P] and (15)N[(31)P] experiments on the same labeled MLVs show that on partitioning into the bilayer, the peptide chains remain in contact with lipid headgroups. The amphipathic lysine side chains of K3 in particular appear to play a key role in the electrostatic interactions with the acidic lipid headgroups. In addition to the extensive peptide-headgroup contact, (13)C[(19)F] REDOR experiments on MLVs containing specifically (19)F-labeled lipid tails suggest that a portion of the peptide is surrounded by a large number of lipid acyl chains. Complementary (31)P[(19)F] REDOR experiments on these MLVs show an enhanced headgroup-lipid tail contact resulting from the presence of K3. Despite these distortions, static (31)P NMR lineshapes indicate that the lamellar structure of the membrane is preserved.

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Citations

Feb 12, 2011·European Biophysics Journal : EBJ·Ralf HeinzmannAnne S Ulrich
Aug 27, 2009·Proceedings of the National Academy of Sciences of the United States of America·Wei QiangDavid P Weliky
Aug 30, 2011·Proceedings of the National Academy of Sciences of the United States of America·Anat Akiva-TalAsher Schmidt
Dec 18, 2013·Biochimica Et Biophysica Acta·Daniel Huster
Apr 22, 2014·Biophysical Chemistry·Eric A SmithPhoebe K Dea
Dec 6, 2011·Annual Review of Physical Chemistry·Mei HongFanghao Hu
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Jul 3, 2017·The Journal of Chemical Physics·Mukul G JainP K Madhu
Feb 29, 2016·Amino Acids·Patricio Carvajal-RondanelliFanny Guzmán
Jul 13, 2017·International Journal of Molecular Sciences·Jae-Ho LeeYounkee Paik
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Feb 8, 2007·Journal of the American Chemical Society·Eszter CzinkiWesley D Allen
Oct 26, 2021·Chemical Reviews·Alexander A ShcherbakovMei Hong

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