Secondary structure and orientation of the pore-forming toxin lysenin in a sphingomyelin-containing membrane

Biochimica Et Biophysica Acta
Monika HerećWiesław I Gruszecki

Abstract

Lysenin is a sphingomyelin-recognizing toxin which forms stable oligomers upon membrane binding and causes cell lysis. To get insight into the mechanism of the transition of lysenin from a soluble to a membrane-bound form, surface activity of the protein and its binding to lipid membranes were studied using tensiometric measurements, Fourier-transform infrared spectroscopy (FTIR) and FTIR-linear dichroism. The results showed cooperative adsorption of recombinant lysenin-His at the argon-water interface from the water subphase which suggested self-association of lysenin-His in solution. An assembly of premature oligomers by lysenin-His in solution was confirmed by blue native gel electrophoresis. When a monolayer composed of sphingomyelin and cholesterol was present at the interface, the rate of insertion of lysenin-His into the monolayer was considerably enhanced. Analysis of FTIR spectra of soluble lysenin-His demonstrated that the protein contained 27% beta-sheet, 28% aggregated beta-strands, 10% alpha-helix, 23% turns and loops and 12% different kinds of aggregated forms. In membrane-bound lysenin-His the total content of alpha-helices, turns and loops, and beta-structures did not change, however, the 1636cm(-1) beta-sheet b...Continue Reading

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Citations

Aug 24, 2013·Toxins·Neelanun Sukumwang, Kazuo Umezawa
Sep 13, 2015·Archives of Biochemistry and Biophysics·Emilia ReszczynskaWieslaw I Gruszecki
Nov 6, 2013·TheScientificWorldJournal·Daniel FologeaTyler Clark
Sep 29, 2011·Biochimica Et Biophysica Acta·Daniel FologeaGreg J Salamo
Dec 19, 2009·Biochimica Et Biophysica Acta·Magdalena KulmaAndrzej Sobota
Apr 21, 2016·Biochimica Et Biophysica Acta·Marta Palusińska-SzyszMałgorzata Cytryńska
May 1, 2012·Cellular Signalling·Magdalena KulmaAndrzej Sobota
Nov 22, 2016·PloS One·Stefan WallnerGerd Schmitz
Apr 7, 2016·Nature Communications·Monika Bokori-BrownChristos G Savva
Aug 9, 2019·Scientific Reports·Radwan Al FaouriGregory Salamo
Apr 23, 2009·Langmuir : the ACS Journal of Surfaces and Colloids·Wiesław I GruszeckiZygmunt Gryczynski
Dec 18, 2018·ACS Omega·Anirban Paul, Divesh N Srivastava

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