PMID: 8457576Mar 30, 1993Paper

Secondary structure and temperature behavior of the acetylcholine receptor by Fourier transform infrared spectroscopy

Biochemistry
D NaumannF Hucho

Abstract

Fourier transform infrared spectroscopy (FT-IR) was used to test the secondary structure of purified acetylcholine receptor membranes from Torpedo californica. The secondary structure was estimated using the spectral features observed in the structure sensitive region of amide I and amide I' (between 1600 and 1700 cm-1), taking advantage of Fourier self-deconvolution and second-derivative techniques along with least-squares band fitting procedures. At least six different amide I' band components could be resolved in D2O and were tentatively assigned to beta-structures (1680 and 1636 cm-1), alpha-helices (1657 cm-1), aperiodic structures and/or distorted helices (1646-1648 cm-1), and turns (1690 and 1668 cm-1), respectively. The beta-band around 1637 cm-1, in particular, turned out to be complex since it reproducibly exhibited weak features near 1630 and 1627 cm-1, thereby suggesting the presence of different chain interacting beta-structures. The band near 1657 cm-1 was assigned to alpha-helices which transverse the membrane bilayers, while 1646-1648-cm-1 component was tentatively attributed to aperiodic structures and alpha-helices localized within the "globular head" of the receptor protein protruding from the membrane surfac...Continue Reading

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Citations

Dec 22, 1999·Progress in Biophysics and Molecular Biology·J L Arrondo, F M Goñi
Feb 17, 2001·European Journal of Biochemistry·J García-GarcíaS Corbalán-García
Aug 1, 1996·European Journal of Biochemistry·F HuchoJ Machold
Apr 1, 1995·Journal of Pharmaceutical Sciences·A DongJ F Carpenter
Mar 10, 2012·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Filiz KorkmazWerner Mäntele
Jun 22, 2010·Journal of Molecular Biology·Filiz Korkmaz-OzkanOzkan Yildiz
Mar 6, 1997·Biochemical and Biophysical Research Communications·G D SockalingumM Manfait
Feb 27, 2001·Biophysical Journal·J M CaaveiroJ M González-Mañas
May 1, 2001·The Journal of Biological Chemistry·N MéthotJ E Baenziger
Apr 21, 1995·The Journal of Biological Chemistry·S BañuelosG Pifat
Feb 7, 2014·Biochemistry·Beatrice N MarkiewiczFeng Gai
Sep 8, 2004·Langmuir : the ACS Journal of Surfaces and Colloids·Per RiglerHorst Vogel

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