PMID: 1998726Jan 29, 1991Paper

Secondary structure of Escherichia coli glucosamine-6-phosphate deaminase from amino acid sequence and circular dichroism spectroscopy

Biochimica Et Biophysica Acta
M M AltamiranoM L Calcagno

Abstract

The secondary structure of the purified glucosamine-6-phosphate deaminase from Escherichia coli K12 was investigated by both circular dichroism (CD) spectroscopy and empirical prediction methods. The enzyme was obtained by allosteric-site affinity chromatography from an overproducing strain bearing a pUC18 plasmid carrying the structural gene for the enzyme. From CD analysis, 34% of alpha-helix, 9% of parallel beta-sheet, 11% of antiparallel beta-sheet, 15% turns and 35% of non-repetitive structures, were estimated. A joint prediction scheme, combining six prediction methods with defined rules using several physicochemical indices, gave the following values: alpha-helix, 37%; beta-sheet, 22%; turns, 18% and coil, 23%. The structure predicted showed also a considerable degree of alternacy of alpha and beta structures; 64% of helices are amphipathic and 90% of beta-sheets are hydrophobic. Overall, the data suggest that deaminase has as dominant motif, an alpha/beta structure.

References

Jun 1, 1979·Biophysical Journal·P Y Chou, G D Fasman
Jan 1, 1978·Annual Review of Biochemistry·P Y Chou, G D Fasman
Mar 15, 1989·Biochemical and Biophysical Research Communications·R S CármenesJ M Martín
Mar 1, 1989·Archives of Biochemistry and Biophysics·M M AltamiranoM L Calcagno
Oct 1, 1989·Molecular & General Genetics : MGG·A P Vogler, J W Lengeler
Oct 1, 1987·Archives of Biochemistry and Biophysics·M M AltamiranoM L Calcagno
Sep 1, 1988·Protein Engineering·V BiouJ Garnier
Jan 1, 1988·Annual Review of Biophysics and Biophysical Chemistry·G E Schulz
Jun 17, 1988·Science·J S Richardson, D C Richardson
Aug 1, 1988·Computer Applications in the Biosciences : CABIOS·O Gascuel, J L Golmard
Jun 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·T P Hopp, K R Woods
Jan 1, 1984·Annual Review of Biochemistry·C Chothia
Mar 15, 1984·Journal of Molecular Biology·W R Taylor, J M Thornton
Jul 15, 1982·Nature·J M Thornton, B L Chakauya
May 5, 1982·Journal of Molecular Biology·J Kyte, R F Doolittle
Sep 1, 1982·Analytical Biochemistry·J P Hennessey, W C Johnson
Mar 3, 1981·Biochemistry·J P Hennessey, W C Johnson

Citations

Oct 12, 2001·Archives of Biochemistry and Biophysics·Ismael Bustos-Jaimes, M L Calcagno
Apr 15, 1997·Proceedings of the National Academy of Sciences of the United States of America·M M AltamiranoA R Fersht
Sep 1, 1992·Archives of Biochemistry and Biophysics·R Lara-LemusM L Calcagno
Oct 17, 1998·Biochimica Et Biophysica Acta·R Lara-Lemus, M L Calcagno

Related Concepts

Related Feeds

Cajal Bodies & Gems

Cajal bodies or coiled bodies are dense foci of coilin protein. Gemini of Cajal bodies, or gems, are microscopically similar to Cajal bodies. It is believed that Cajal bodies play important roles in RNA processing while gems assist the Cajal bodies. Find the latest research on Cajal bodies and gems here.