Secondary structures and features of the 18S, 5.8S and 26S ribosomal RNAs from the Apicomplexan parasite Toxoplasma gondii

Gene
S GagnonR C Levesque

Abstract

The two major subunits of the ribosomal RNA (rRNA) of Toxoplasma gondii, 18S and 26S, as well as 5.8S, have been sequenced and folded according to known consensus and established secondary structures. Conserved and variable nucleotide (nt) regions were identified using multiple alignments with rRNA sequences of selected organisms. The 18S rRNA showed a well conserved core structure of 48 stems and a hypervariable V4 region identified four additional stems including a pseudoknot. The 18S rRNA contained an additional helix in the V2 region located between nt 204 to 258. We noted that T. gondii 18S does not have a true V6 region, but was organized as a motif of a simple stem. T. gondii 26S had a conserved core structure of 83 stems and its expansion segments, so-called divergent domains, demonstrated a high degree of similarity with secondary structures from rRNA of dinoflagellates and ciliates. For the T. gondii 26S sequence, we found two additional stems, D3d and D3e, composed of 140 nt having a higher deltaG value. These segments are absent from the prokaryotic rRNA structures, whereas the hypervariable V4 region of the small subunit is not as variable. The well preserved structures could indicate an additional function for the...Continue Reading

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Citations

Mar 12, 2004·International Journal for Parasitology·David A MorrisonJens G Mattsson
Apr 14, 2005·International Journal for Parasitology·David A Morrison
Apr 18, 2001·International Journal for Parasitology·J R SlapetaJ Lukes
Sep 28, 2002·International Journal for Parasitology·Odile Mercereau-PuijalonEmmanuel Bischoff
Mar 23, 2002·The Journal of Eukaryotic Microbiology·Naomi M FastPatrick J Keeling
Aug 20, 2005·The Journal of Parasitology·Mark D SchrenzelBruce A Rideout
Mar 6, 2016·Journal of Microbiological Methods·Elke T VermeulenMichelle L Power
Jul 22, 2014·Journal of Cellular Biochemistry·Farah R ShakooriAbdul Rauf Shakoori
Nov 26, 2009·FEMS Microbiology Reviews·Ana Lilia Torres-MachorroImelda López-Villaseñor
Apr 15, 2008·Molecular Phylogenetics and Evolution·Niamh E Redmond, Grace P McCormack
Feb 6, 2019·Non-coding RNA·Marine LambertPatrick Provost
Oct 6, 2000·Molecular Biology and Evolution·B BilloudJ S Deutsch
Sep 6, 2001·The Journal of Parasitology·S L Perkins, A K Keller

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