PMID: 9557696Apr 29, 1998Paper

Secondary structures in the capsid protein coding sequence and 3' nontranslated region involved in amplification of the tobacco etch virus genome

Journal of Virology
R Haldeman-CahillJ C Carrington

Abstract

The 3'-terminal 350 nucleotides of the tobacco etch potyvirus (TEV) genome span the end of the capsid protein (CP)-coding sequence and the 3' nontranslated region (NTR). The CP-coding sequence within this region contains a 105-nucleotide cis-active element required for genome replication (S. Mahajan, V. V. Dolja, and J. C. Carrington, J. Virol. 70:4370-4379, 1996). To investigate the sequence and secondary structure requirements within the CP cis-active region and the 3' NTR, a systematic linker-scanning mutagenesis analysis was done. Forty-six mutations, each with two to six nucleotide substitutions, were introduced at consecutive hexanucleotide positions in the genome of a recombinant TEV strain expressing a reporter protein (beta-glucuronidase). Genome amplification activity of each mutant in the protoplast cell culture system was measured. Mutations that severely debilitated genome amplification were identified throughout the CP-coding cis-active sequence and at several distinct locations within the 3' NTR. However, based on a computer model of RNA folding, mutations that had the most severe effects mapped to regions that were predicted to form base-paired secondary structures. Linker-scanning mutations predicted to affect ...Continue Reading

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Citations

Apr 22, 2005·The Plant Journal : for Cell and Molecular Biology·Byoung-Cheorl KangMolly M Jahn
Mar 28, 2009·Biology Letters·Rafael SanjuánSantiago F Elena
Apr 12, 2014·Frontiers in Plant Science·Kristiina Mäkinen, Anders Hafrén
Apr 9, 2015·Genome Biology·Rachel S SealfonPardis C Sabeti
Apr 12, 2014·The Journal of General Virology·K I IvanovK Mäkinen
Jul 18, 2002·Molecular Plant-microbe Interactions : MPMI·Jari P T ValkonenTuija Kekarainen
Oct 7, 2006·Virus Genes·Alice Kazuko Inoue-NagataTatsuya Nagata
Jan 24, 2014·Journal of Virology·Eszter MajerJosé-Antonio Daròs
Jan 19, 2010·Molecular Plant Pathology·Benoit Moury
Jul 25, 2000·Journal of Virology·M VarrelmannE Maiss
Feb 16, 2018·Journal of Virology·Araiz GalloJuan Antonio García

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