Secondary typing of Mycobacterium tuberculosis isolates with matching IS6110 fingerprints from different geographic regions of the United States

Journal of Clinical Microbiology
Z YangM D Cave

Abstract

Fifty-nine isolates of Mycobacterium tuberculosis obtained from different states in the United States and representing 25 interstate clusters were investigated. These clusters were identified by computer-assisted analysis of DNA fingerprints submitted during 1996 and 1997 by different laboratories participating in the CDC National Genotyping and Surveillance Network. Isolates were fingerprinted with the IS6110 right-hand probe (IS6110-3'), the IS6110 left-hand probe (IS6110-5'), and the probe pTBN12, containing the polymorphic GC-rich sequence (PGRS). Spoligotyping based on the polymorphism in the 36-bp direct-repeat locus was also performed. As a control, 43 M. tuberculosis isolates in 17 clusters obtained from patients in Arkansas during the study period were analyzed. Of the 25 interstate clusters, 19 were confirmed as correctly clustered when all the isolates were analyzed on the same gel using the IS6110-3' probe. Of the 19 true IS6110-3' clusters, 10 (53%) were subdivided by one or more secondary typing methods. Clustering of the control group was virtually identical by all methods. Of the three different secondary typing methods, spoligotyping was the least discriminating. IS6110-5' fingerprinting was as discriminating a...Continue Reading

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Citations

Oct 17, 2006·Clinical Microbiology Reviews·Barun MathemaBarry N Kreiswirth
Jul 2, 2002·American Journal of Respiratory and Critical Care Medicine·Stephen E WeisPeter F Barnes
Mar 4, 2011·Future Microbiology·Midori Kato-MaedaLaura Flores
Mar 26, 2008·Revista portuguesa de pneumologia·Isabel PortugalLaura Brum
Dec 3, 2014·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·Sara ThabetHelmi Mardassi
May 19, 2016·Revista Da Sociedade Brasileira De Medicina Tropical·Seifu Gizaw FeyisaMohammad Mehdi Feizabadi
Jul 7, 2007·Memórias do Instituto Oswaldo Cruz·Maraníbia C OelemannPhilip N Suffys
Jan 16, 2004·Scandinavian Journal of Infectious Diseases·Manca Zolnir-DovcJurij Sorli

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