Secondhand smoke inhibits both Cl- and K+ conductances in normal human bronchial epithelial cells.

Respiratory Research
Amy N SavitskiJames L Kreindler

Abstract

Secondhand smoke (SHS) exposure is an independent risk factor for asthma, rhinosinusitis, and more severe respiratory tract infections in children and adults. Impaired mucociliary clearance with subsequent mucus retention contributes to the pathophysiology of each of these diseases, suggesting that altered epithelial salt and water transport may play an etiological role. To test the hypothesis that SHS would alter epithelial ion transport, we designed a system for in vitro exposure of mature, well-differentiated human bronchial epithelial cells to SHS. We show that SHS exposure inhibits cAMP-stimulated, bumetanide-sensitive anion secretion by 25 to 40% in a time-dependent fashion in these cells. Increasing the amount of carbon monoxide to 100 ppm from 5 ppm did not increase the amount of inhibition, and filtering SHS reduced inhibition significantly. It was determined that SHS inhibited cAMP-dependent apical membrane chloride conductance by 25% and Ba2+-sensitive basolateral membrane potassium conductance by 50%. These data confirm previous findings that cigarette smoke inhibits chloride secretion in a novel model of smoke exposure designed to mimic SHS exposure. They also extend previous findings to demonstrate an effect on ba...Continue Reading

References

Jun 1, 1983·The Journal of Clinical Investigation·M J Welsh
Jan 1, 1996·American Journal of Respiratory Cell and Molecular Biology·T E GrayP Nettesheim
Apr 13, 1999·The American Journal of Physiology·D C Devor, J M Pilewski
May 6, 1999·The Journal of General Physiology·D C DevorR J Bridges
Sep 6, 2000·American Journal of Respiratory Cell and Molecular Biology·M MallK Kunzelmann
Oct 12, 2000·JAMA : the Journal of the American Medical Association·X WangG R Cutting
Jul 13, 2002·American Journal of Physiology. Lung Cellular and Molecular Physiology·Stephen T BallardSarah K Inglis
Jan 24, 2004·The European Respiratory Journal·R C Boucher
Feb 24, 2004·Annual Review of Physiology·Xiang Dong TangToshinori Hoshi
Mar 16, 2004·Environmental Research·Wan-Kuen JoJong-In Dong
Jul 30, 2004·Thorax·H van der VaartN H T ten Hacken
Aug 19, 2004·Respiration; International Review of Thoracic Diseases·Christoph BeisswengerRobert Bals
Nov 17, 2004·Proceedings of the National Academy of Sciences of the United States of America·Lisa M DiPilatoJin Zhang
Jan 1, 2005·American Journal of Physiology. Lung Cellular and Molecular Physiology·James L KreindlerHenry Danahay
Jan 20, 2005·The Journal of General Physiology·Wei WangKevin L Kirk
Sep 16, 2005·American Journal of Physiology. Cell Physiology·André M CantinJohn W Hanrahan
Oct 18, 2005·American Journal of Physiology. Lung Cellular and Molecular Physiology·Henry DanahayRobert J Bridges
Feb 25, 2006·American Journal of Respiratory and Critical Care Medicine·André M CantinPeter Durie
Oct 19, 2006·American Journal of Preventive Medicine·Vaughan W Rees, Gregory N Connolly
Apr 21, 2007·American Journal of Respiratory and Critical Care Medicine·Robin R DeterdingUNKNOWN Cystic Fibrosis Therapeutics Development Network and the Inspire 08-103 Working Group
May 30, 2007·American Journal of Preventive Medicine·Kenneth P Moritsugu
Sep 28, 2007·Environmental Health : a Global Access Science Source·Jon O EbbertRichard D Hurt
Dec 15, 2007·Journal of Pediatric Psychology·Georg E MattMelbourne F Hovell
Apr 10, 2008·Journal of Analytical Toxicology·Lucy A ClunesRobert Tarran
Jun 24, 2008·The Journal of Allergy and Clinical Immunology·Mang YuKent E Pinkerton
Oct 11, 2008·Free Radical Biology & Medicine·Christian SchwarzerBeate Illek
Jan 7, 2009·The Journal of Biological Chemistry·Ken AsadaTetsushi Furukawa
Mar 18, 2009·The Journal of General Physiology·Carol A BertrandRaymond A Frizzell
Apr 15, 2009·The Journal of Biological Chemistry·Wan NamkungA S Verkman

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Citations

Dec 24, 2010·Current Opinion in Pulmonary Medicine·Andreas D Flouris, Yiannis Koutedakis
Oct 4, 2011·American Journal of Physiology. Lung Cellular and Molecular Physiology·Ke-Qing ZhaoJames L Kreindler
Mar 26, 2013·American Journal of Respiratory Cell and Molecular Biology·Charles A DownsMy N Helms
Oct 9, 2012·The Journal of Clinical Investigation·Robert J LeeNoam A Cohen
Jan 22, 2011·PloS One·Natalia Goldstein-DaruechNoam A Cohen
Feb 18, 2014·The Journal of Clinical Investigation·Robert J LeeNoam A Cohen
Oct 11, 2013·American Journal of Respiratory Cell and Molecular Biology·James A LambertSteven M Rowe
Feb 13, 2014·Respiratory Research·S Vamsee RajuSteven M Rowe
Nov 4, 2015·Frontiers in Microbiology·Srinivasan Chinnapaiyan, Hoshang J Unwalla
Jul 1, 2014·American Journal of Respiratory Cell and Molecular Biology·Hoshang J UnwallaMatthias Salathe
Jun 17, 2016·International Forum of Allergy & Rhinology·Nithin D AdappaNoam A Cohen
Mar 3, 2012·Otolaryngology--head and Neck Surgery : Official Journal of American Academy of Otolaryngology-Head and Neck Surgery·Mehmet HabesogluCagatay Oysu
May 19, 2012·Otolaryngology--head and Neck Surgery : Official Journal of American Academy of Otolaryngology-Head and Neck Surgery·Marcelo B AntunesNoam A Cohen
May 12, 2018·Expert Review of Respiratory Medicine·Elena Fernandez FernandezCatherine M Greene
Dec 24, 2018·American Journal of Respiratory Cell and Molecular Biology·Vivian Y LinSteven M Rowe
Oct 6, 2017·American Journal of Physiology. Cell Physiology·Francis H WongJohn W Hanrahan
Mar 21, 2020·Respiratory Research·Lawrence W RasmussenS Vamsee Raju
Oct 8, 2020·Journal of Cystic Fibrosis : Official Journal of the European Cystic Fibrosis Society·Elizabeth BakerGabriela R Oates

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