Abstract
The emerging role of bone morphogenetic proteins (BMPs) in the initiation and progression of multiple cancers has drawn great attention in cancer research. In this study, we report that BMP-2 can promote the proliferation of the pancreatic tumor cell line, PANC-1. Secreted phosphoprotein 24 kD (Spp24), a BMP binding protein, did not affect the proliferation of the cells but promoted the apoptosis of the cells in vitro. In a xeneograft tumor model using PANC-1 cells, BMP-2 dramatically promoted tumor growth, while Spp24 not only abolished the effect of BMP-2, but also dramatically induced tumor shrinking when used alone. Activation of Smad1/5/8 participated in this process as demonstrated by immunohistochemical staining of phosphorylated Smad 1/5/8. We conclude that Spp24 can be developed into a therapeutic agent that could be employed in clinical situations where the inhibition of BMPs and related proteins is advantageous.
References
Aug 1, 1997·Nature Medicine·A H Reddi
Apr 30, 1999·Gastroenterology·J KleeffM Korc
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Jun 24, 2003·Carcinogenesis·Elaine M LangenfeldJohn Langenfeld
Mar 31, 2004·Lancet·Donghui LiJames L Abbruzzese
Nov 19, 2004·Cancer Research·Dominik R HaudenschildA Hari Reddi
Dec 21, 2004·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Keyvan BehnamSamuel S Murray
Aug 30, 2005·Bone·Brian T FeeleyJay R Lieberman
Oct 26, 2005·Oncogene·E M LangenfeldJ Langenfeld
Nov 19, 2005·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Brian T FeeleyJay R Lieberman
Dec 28, 2006·Scandinavian Journal of Gastroenterology·Daniel NeureiterMatthias Ocker
Apr 12, 2007·BMC Genomics·Prathibha RanganathanPaturu Kondaiah
Dec 14, 2007·Connective Tissue Research·Elsa J Brochmann MurrayKeyvan Behnam
Feb 7, 2008·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Chananit SintuuElsa J Brochmann
Jan 1, 2010·Neurosurgery·Jayesh P ThawaniPaul Park
Feb 18, 2010·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Elsa J BrochmannSamuel S Murray
Sep 18, 2010·Pathology Oncology Research : POR·Xiong ChenWeiJia Sun
Jan 22, 2011·Pharmacoepidemiology and Drug Safety·Daniel MinesGregory S Cooper
Jun 28, 2011·Experimental Cell Research·Siru VirtanenAnne Kallioniemi
Aug 31, 2013·PloS One·Haijun TianSamuel S Murray
Jan 28, 2014·Hepatobiliary & Pancreatic Diseases International : HBPD INT·Xu LiuChun-Yan Wu
Sep 30, 2014·The Spine Journal : Official Journal of the North American Spine Society·Haijun TianMichael D Daubs
Jan 7, 2015·CA: a Cancer Journal for Clinicians·Rebecca L SiegelAhmedin Jemal
Feb 11, 2015·Carbohydrate Polymers·Peipei WangKan Ding
Citations
Jul 13, 2016·Stem Cells Translational Medicine·Dmitriy SheynZulma Gazit
Jan 23, 2017·Cytokine & Growth Factor Reviews·Haijun TianSamuel S Murray
Feb 10, 2019·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Haijun TianSamuel S Murray
Dec 19, 2019·BMC Cancer·Yaqin TuGuorun Fan
Sep 24, 2016·Oncotarget·Lijie ZhangJinpu Yu
Feb 14, 2019·PeerJ·Xiuzhi ZhangLiping Dai
Jan 23, 2021·Calcified Tissue International·Mandy E TurnerRachel M Holden
Feb 5, 2021·Cancer Management and Research·Juncheng GuoJinfang Zheng
Jul 26, 2018·Journal of Proteomics·Linpei ZhangYouhe Gao
Jul 18, 2018·Journal of Proteome Research·Chengjian TuYue Wu