Secreted protein acidic, rich in cysteine induces pulp cell migration via alphavbeta3 integrin and extracellular signal-regulated kinase

Oral Diseases
P Pavasant, T Yongchaitrakul

Abstract

The aim of this study was to investigate the influence of secreted protein acidic, rich in cysteine (SPARC) on the migration of human dental pulp (HDP) cells. Secreted protein acidic, rich in cysteine was applied in the lower chamber of the chemotaxis apparatus and migration was determined by counting the cells that migrated through the membrane. To determine the signaling pathway involved, cells were incubated with inhibitors for 30 min prior to the migration assay. The results indicated that SPARC induced HDP cell migration in a dose-dependent manner via extracellular signal-regulated kinase (ERK). The migration could be inhibited both by the anti-alphavbeta3 integrin antibody and by suramin, a non-selective growth factor receptor and G-protein coupled receptor antagonists. The anti-alphavbeta3 integrin antibody could also inhibit ERK activation, suggesting the possible role of alphavbeta3 integrin on the regulation of ERK and cell migration. Interestingly, both suramin and SB225002, another G-protein coupled receptor antagonist, suppressed ERK activation. Secreted protein acidic, rich in cysteine could act as a chemotactic factor and facilitate migration, possibly through the G-protein coupled receptor, alphavbeta3 integrin ...Continue Reading

References

May 1, 1992·Calcified Tissue International·T ReichertL W Fisher
Jul 1, 1987·Journal of Cellular Physiology·R J CoffeyH L Moses
Jul 1, 1994·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·T Takano-YamamotoS Nomura
Sep 1, 1996·The Journal of Cell Biology·A HuttenlocherA F Horwitz
Apr 16, 1998·European Journal of Oral Sciences·D Tziafas, S Papadimitriou
Oct 29, 1998·Biochemical and Biophysical Research Communications·B A YoungS E Goldblum
May 9, 2001·The Journal of Clinical Investigation·A D Bradshaw, E H Sage
Oct 24, 2002·The Journal of Biological Chemistry·Marnie S RobertsJim C Norman
Mar 19, 2003·Advances in Dental Research·H MagloireJ C Farges
May 15, 2003·Endocrinology·Anne M DelanyErnesto Canalis
Jun 26, 2003·Experimental Eye Research·Bridgette L BerryhillJohn R Hassell
Jul 23, 2003·Proceedings of the National Academy of Sciences of the United States of America·Melanie RolliBrunhilde Felding-Habermann
Jul 30, 2003·The Journal of Biological Chemistry·Sarmishtha DeTatiana V Byzova
Sep 17, 2004·Journal of Cell Science·Cai HuangMichael D Schaller
Jan 1, 2005·The Journal of Pharmacology and Experimental Therapeutics·Wen-Cheng Chung, John C Kermode
Jan 18, 2006·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Rong Xue WuOliver Ritter
May 4, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Julia KzhyshkowskaE Helene Sage

❮ Previous
Next ❯

Citations

Jun 23, 2010·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Jessica M Trombetta, Amy D Bradshaw
Mar 5, 2014·The Journal of Clinical Investigation·Houda AlachkarGuido Marcucci
Aug 9, 2011·Cellular and Molecular Life Sciences : CMLS·Lee B RiveraRolf A Brekken
Jun 17, 2011·Advances in Dental Research·M Goldberg
Nov 20, 2013·Laboratory Investigation; a Journal of Technical Methods and Pathology·Jelena Grahovac, Alan Wells
Dec 5, 2009·Seminars in Cell & Developmental Biology·Alexandre Chlenski, Susan L Cohn

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.

Anthelmintics

Anthelmintics or antihelminthics are a group of antiparasitic drugs that expel parasitic worms (helminths) and other internal parasites from the body by either stunning or killing them and without causing significant damage to the host. Discover the latest research on anthelmintics here.

African Trypanosomiasis

African trypanosomiasis, also known as sleeping sickness, is an insect-borne parasitic disease of humans and other animals. It is caused by protozoa of the species Trypanosoma brucei and almost invariably progresses to death unless treated. Discover the latest research on African trypanosomiasis here.

Anthelmintics (ASM)

Anthelmintics or antihelminthics are a group of antiparasitic drugs that expel parasitic worms (helminths) and other internal parasites from the body by either stunning or killing them and without causing significant damage to the host. Discover the latest research on anthelmintics here.

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.