Secreted subtilisin gene family in Trichophyton rubrum

Gene
Olivier JoussonM Monod

Abstract

Secreted proteases constitute potential virulence factors of dermatophytes. A total of seven genes encoding putative serine proteases of the subtilisin family (SUB) were isolated in Trichophyton rubrum. Based on sequence data and intron-exon structure, a phylogenetic analysis of subtilisins from T. rubrum and other fungi revealed a presumed ancestral lineage comprising T. rubrum SUB2 and Aspergillus SUBs. All other SUBs (SUB1, SUB3-7) are dermatophyte-specific and have apparently emerged more recently, through successive gene duplication events. We showed that two subtilisins, Sub3 and Sub4, were detected in culture supernatants of T. rubrum grown in a medium containing soy protein as a sole nitrogen source. Both recombinant enzymes produced in Pichia pastoris are highly active on keratin azure suggesting that these proteases play an important role in invasion of keratinised tissues by the fungus. The set of deduced amino acid sequences of T. rubrum SUB ORFs allowed the identification of orthologous Subs secreted by other dermatophyte species using proteolysis and mass spectrometry.

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Citations

Oct 31, 2006·Medical Mycology·Monica S Ferreira-NozawaNilce M Martinez-Rossi
Jul 6, 2011·Molecular Biology and Evolution·Anna MuszewskaMarcin Grynberg
Mar 5, 2008·Current Opinion in Infectious Diseases·Bernard MignonSandy Vermout
May 18, 2010·Applied and Environmental Microbiology·Karine SalaminMichel Monod
Jun 10, 2008·Eukaryotic Cell·Theodore C WhiteMatthew R Henn
Apr 7, 2006·Journal of Clinical Microbiology·V KardjevaY Gräser
Mar 1, 2014·Medical Mycology·Emily Whiston, John W Taylor
Dec 17, 2009·Microbial Pathogenesis·Jian ChenWei Lai
Jan 29, 2013·The Journal of Investigative Dermatology·Maria GrumbtPeter Staib
Jun 26, 2007·Microbial Pathogenesis·Fernanda C A MaranhãoNilce M Martinez-Rossi
Aug 11, 2015·Evidence-based Complementary and Alternative Medicine : ECAM·Tatiana Takahasi KomotoAna Lúcia Fachin
Mar 13, 2015·FEMS Microbiology Letters·Chitra LatkaBhupesh Taneja
Dec 4, 2008·FEMS Immunology and Medical Microbiology·Sandy VermoutBernard Mignon
Dec 13, 2006·FEMS Microbiology Letters·Karin GiddeyMichel Monod
Aug 8, 2007·FEMS Microbiology Letters·Sandy VermoutBernard Mignon
Mar 15, 2018·Medical Mycology·Yvonne GräserSybren de Hoog
Jan 24, 2018·Medical Mycology·Derry K Mercer, Colin S Stewart
Jun 14, 2019·Journal of Clinical Microbiology·Huilin SuSybren de Hoog
May 15, 2008·Mycopathologia·Sandy VermoutBernard Mignon
Sep 4, 2016·Mycopathologia·Nilce M Martinez-RossiAntonio Rossi
May 15, 2008·Mycopathologia·Michel Monod

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