PMID: 11316259Apr 24, 2001Paper

Secretin stimulates HCO3(-) and acetate efflux but not Na+/HCO3(-) uptake in rat pancreatic ducts

Pflügers Archiv : European journal of physiology
I Novak, B C Christoffersen

Abstract

Pancreatic ducts secrete HCO3(-), but transport mechanisms are unresolved and possibly vary between species. Our aim was to study the intracellular pH (pHi) regulation and thus H+/HCO3- transport in rat pancreatic ducts. Of particular interest was the Na+/HCO3(-) cotransporter, thought to be important in HCO3(-) -transporting epithelia. pHi was measured with BCECF in freshly isolated intralobular ducts. A reduction in extracellular Na+ concentration or application of HOE 694 (1 microM) decreased pHi by 0.1 to 0.6 pH units, demonstrating Na+/H+ exchanger activity. A reduction in extracellular Cl- concentration or addition of H2DIDS (10 microM) increased pHi by 0.1 to 0.5 pH units, demonstrating Cl-/ HCO(3)- (OH ) exchanger activity. In experimental acidosis, extracellular HCO3(-)/CO2 buffer did not increase the rate of pHi recovery, indicating that provision of HCO3(-) by the Na+/HCO3(-) cotransporter was not apparent. Most importantly, Na+/HCO3(-) cotransport was not stimulated by secretin (1 nM). In contrast, in experimental alkalosis the pHi recovery was increased in HCO3(-)/CO2 buffer, possibly due to Na+/HCO3(-) cotransport in the efflux mode. Secretin (1 nM) and carbachol (1 microM) stimulated HCO3(-) efflux, which can acc...Continue Reading

Citations

Jan 22, 2004·Biochemical and Biophysical Research Communications·Eleni RoussaFrank Thévenod
Oct 28, 2010·The Journal of Biological Chemistry·Ivana NovakSusanne E Hede
Oct 31, 2002·The Journal of General Physiology·H IshiguroS Naruse
Feb 16, 2005·Annual Review of Physiology·Martin C StewardR Maynard Case
Nov 6, 2014·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Su Chii KongStine Falsig Pedersen
Dec 31, 2004·American Journal of Physiology. Cell Physiology·Péter HegyiBarry E Argent
Apr 21, 2005·Pancreas·Seth R HootmanDouglas B Luckie
Aug 12, 2005·American Journal of Physiology. Cell Physiology·Elena V BouzinovaChristian Aalkjaer
Apr 4, 2003·American Journal of Physiology. Cell Physiology·Peter HegyiBarry E Argent
Oct 19, 2019·Bulletin of Experimental Biology and Medicine·M A VodyanovaO V Mislavsky
Mar 8, 2006·World Journal of Gastroenterology : WJG·Zoltan RakonczayBarry E Argent
Nov 8, 2002·Biochimica Et Biophysica Acta·Ivana Novak, Mette R Hansen

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