Secretion expression of recombinant glucagon inEscherichia coli

Science in China. Series C, Life Sciences
Chongwei WenS Zhu

Abstract

A novel approach for the preparation of recombinant human glucagon was described. An expression vector pAGluT, containing phoA promoter, phoA signal peptide and glucagon gene, was constructed by means of genetic engineering.Escherichia coli strain YK537 was transformed with pAGluT. High-level secretory expression of recombinant human glucagon was achieved. The expression yield of recombinant human glucagon was found to be 80 mg/L, approximately 30% of the total proteins in supernatant. The biological activities and the physicochemical properties of the purified recombinant human glucagon were found to be the same as that of native glucagon. In addition, our results suggested that phoA expression system may be suitable for the expression of other small peptides.

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Citations

Apr 9, 2015·AMB Express·Angelina M M BassoMaria Fatima Grossi-de-Sa
Oct 21, 2018·World Journal of Microbiology & Biotechnology·Zhuolin QiuXiangsheng Cai

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