Secretion of a bacterial protein by mammalian cells

Journal of Biotechnology
J M Clément, M Jehanno

Abstract

The MalE protein is a periplasmic maltooligosaccharide binding protein from Escherichia coli. This protein is widely used as a model for protein export in bacteria and as a vector for the export and one-step affinity purification of foreign polypeptides. Expression of MalE was studied in various animal cell lines. The protein was exported into the culture medium, following the classical pathway of eukaryotic protein secretion. This was shown by a combination of approaches including the use of inhibitors of the Golgi complex and immunocytological methods. The signal sequence of MalE is required for secretion and a specific signal can be added to MalE that targets it to the endoplasmic reticulum. This work opens the way to the study of the secretion of a bacterial protein and to its use as a vector for protein secretion and purification from mammalian cells.

References

Apr 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·A HinnenG R Fink
Jan 2, 1992·Gene·T W ChristiansonP Hieter
Feb 21, 1992·Cell·M J Chrispeels, N V Raikhel
Mar 1, 1992·The Journal of Cell Biology·R D KlausnerJ Lippincott-Schwartz
Dec 1, 1990·Trends in Biochemical Sciences·H R Pelham
Nov 8, 1990·Nature·M J Lewis, H R Pelham
Jan 1, 1989·Annual Review of Cell Biology·H R Pelham
Nov 15, 1986·The Biochemical Journal·F A Marston
Jan 1, 1985·Annual Review of Biochemistry·R Kornfeld, S Kornfeld
Apr 1, 1973·Virology·F L Graham, A J van der Eb
Jun 14, 1984·Nature·M WiedmannT A Rapoport
Mar 1, 1993·Microbiological Reviews·A P Pugsley
Feb 17, 1994·Nature·B Dobberstein
Jun 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·H D BernsteinP Walter

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