Secretion of an Argonaute protein by a parasitic nematode and the evolution of its siRNA guides

Nucleic Acids Research
Franklin Wang-Ngai ChowAmy H Buck

Abstract

Extracellular RNA has been proposed to mediate communication between cells and organisms however relatively little is understood regarding how specific sequences are selected for export. Here, we describe a specific Argonaute protein (exWAGO) that is secreted in extracellular vesicles (EVs) released by the gastrointestinal nematode Heligmosomoides bakeri, at multiple copies per EV. Phylogenetic and gene expression analyses demonstrate exWAGO orthologues are highly conserved and abundantly expressed in related parasites but highly diverged in free-living genus Caenorhabditis. We show that the most abundant small RNAs released from the nematode parasite are not microRNAs as previously thought, but rather secondary small interfering RNAs (siRNAs) that are produced by RNA-dependent RNA Polymerases. The siRNAs that are released in EVs have distinct evolutionary properties compared to those resident in free-living or parasitic nematodes. Immunoprecipitation of exWAGO demonstrates that it specifically associates with siRNAs from transposons and newly evolved repetitive elements that are packaged in EVs and released into the host environment. Together this work demonstrates molecular and evolutionary selectivity in the small RNA sequen...Continue Reading

References

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Citations

Aug 10, 2019·RNA Biology·Lindsay P WinkenbachJulie M Claycomb
Dec 28, 2019·Nucleic Acids Research·José Roberto Bermúdez-BarrientosCei Abreu-Goodger
Dec 18, 2019·Parasitology·Collette BrittonEileen Devaney
Dec 2, 2020·International Journal of Molecular Sciences·Emma LaytonIan S Roberts
Dec 1, 2019·Cancer Research·Alissa M Weaver, James G Patton
Jan 9, 2021·Trends in Biochemical Sciences·Juan Pablo TosarAlfonso Cayota
Oct 28, 2019·Current Opinion in Plant Biology·Qiang CaiHailing Jin
Aug 16, 2019·Cell Host & Microbe·Chien-Yu HuangHailing Jin
Mar 28, 2021·Molecular Immunology·Marcela A CucherLaura Kamenetzky
Jul 11, 2021·Molecular Immunology·Claire Drurey, Rick M Maizels
Jul 6, 2020·International Journal for Parasitology·Peter J TaylorPeter Sarkies
Jul 12, 2020·International Journal for Parasitology·Javier SotilloLucienne Tritten
Jul 14, 2020·International Journal for Parasitology·Claire DrureyRick M Maizels
Jun 3, 2021·Annual Review of Phytopathology·Yongli QiaoWenbo Ma

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Datasets Mentioned

BETA
GSE117169

Methods Mentioned

BETA
RNA-Seq
sRNA-seq
PullSeq
Immunoprecipitations
PCR
transmission electron microscopy
RNAseq
immunoprecipitation

Software Mentioned

Rfam
GenePS
BRAKER
exWAGO
MAFFT
edgeR
Image Studio Lite (
GenomicRanges R package
OrthoFinder
KinFin

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