PMID: 2105719Jan 15, 1990Paper

Secretion of Bacillus subtilis levansucrase. Fe(III) could act as a cofactor in an efficient coupling of the folding and translocation processes

The Biochemical Journal
R ChambertM F Petit-Glatron

Abstract

The refolding of levansucrase denatured by urea was studied as a possible model for the second step of the secretion pathway of this protein. The folding-unfolding transition was monitored by measuring intrinsic fluorescence and resistance to proteolysis. Both methods provided the same estimation for the unfolding free energy of levansucrase, delta GD, which was 30.1 +/- 1.7 kJ.mol-1 (7.2 +/- 0.4 kcal.mol-1) at pH 7 in 0.1 M-potassium phosphate buffer. The rate of refolding was greatly enhanced by Fe3+, whereas the Fe3+ chelator EDTA prevented correct refolding. Fe3+ allowed the protein to reach its folded form in medium in which the dielectric constant had been lowered by ethanol. The efficiency in vivo of the export of levansucrase bearing an amino acid modification which blocks the second step of the translocation pathway was greatly increased by high concentrations of Fe3+ in the culture medium. Assuming that the protein folding governs the second step of the secretion process of levansucrase, we discuss from an irreversible thermodynamic point of view the possible role of Fe3+ in the efficient coupling of the two events.

Citations

May 1, 1991·Molecular Microbiology·V P KontinenM Sarvas
Apr 5, 2012·Molecular Microbiology·Brian M Forster, Hélène Marquis
Nov 18, 2004·Biochimica Et Biophysica Acta·Matti SarvasJan Maarten van Dijl
Apr 23, 1999·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·S HilfikerP Greengard
Jan 7, 2020·Annual Review of Food Science and Technology·Kang ZhangJing Wu
Sep 7, 2000·Microbiology and Molecular Biology Reviews : MMBR·H TjalsmaJ M van Dijl
Jul 2, 1999·Applied and Environmental Microbiology·A BolhuisJ M van Dijl
Mar 1, 1993·The New Phytologist·Nicholas A Jacques

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