PMID: 2497752May 1, 1989Paper

Secretion of cholesteryl ester transfer protein-lipoprotein complexes by human HepG2 hepatocytes

Atherosclerosis
R A FaustJ J Albers

Abstract

We have employed immunoaffinity chromatography to characterize the distribution of cholesteryl ester transfer activity in particles secreted by HepG2 hepatocytes. HepG2-secreted cholesteryl ester transfer activity is associated with apoprotein (apo) A-I (58%) as well as apo A-II (55%), and is not associated with apo B or E. In contrast, our previous studies have shown that most (88%) cholesteryl ester transfer activity in human plasma is associated with apo A-I whereas very little (7%) is associated with apo A-II. Thus, the distribution of cholesteryl ester transfer activity in plasma particles likely reflects active remodeling of nascent particles in the plasma compartment. Further data suggested that HepG2 cells secrete a lipid transfer inhibitor activity which is associated with apo E-containing lipoprotein particles. This inhibitory activity is heat labile.

References

Dec 1, 1975·Metabolism: Clinical and Experimental·J J AlbersW R Hazzard
Sep 28, 1978·Biochimica Et Biophysica Acta·N M PattnaikD B Zilversmit
May 1, 1987·Arteriosclerosis : an Official Journal of the American Heart Association, Inc·R A Faust, J J Albers
Jan 1, 1986·Arteriosclerosis : an Official Journal of the American Heart Association, Inc·O SteinY Stein
Sep 18, 1973·Biochemical and Biophysical Research Communications·L PickartM M Thaler
May 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·V I ZannisJ L Breslow
Oct 4, 1984·Biochimica Et Biophysica Acta·Y S Son, D B Zilversmit
Jan 1, 1984·Arteriosclerosis : an Official Journal of the American Heart Association, Inc·J J AlbersA Steinmetz
Jan 1, 1982·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·Y C Ha, P J Barter

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