Secretion of cumulus expansion-enabling factor (CEEF) in porcine follicles

Molecular Reproduction and Development
Radek ProchazkaJ Motlík

Abstract

The objective of this study was to find out whether porcine cumulus and mural granulosa cells can secrete cumulus expansion-enabling factor (CEEF). Culture drops of M-199 medium were conditioned with denuded porcine oocytes (1 oocyte/microliter), cumulus cells from oocytectomized complexes (1 OOX/microliter), pieces of mural granulosa isolated from preantral to preovulatory follicles (1000 cells/microliter), or oviductal cells (1000 cells/microliter) for 24 hr. The production of CEEF was assessed by the addition of mouse OOX and follicle-stimulating hormone (FSH) (1 microgram/ml) to microdrops of the conditioned medium. After 16-18 hr, expansion of the mouse OOX was scored on a scale of 0 to 4 by morphologic criteria. Mouse OOX did not expand in nonconditioned FSH-supplemented medium. Immature porcine oocytes produced +3 to +4 expansion of the mouse OOX. Granulosa cells isolated from preantral and early antral follicles and cumulus cells isolated from all states of follicle development constitutively secreted CEEF under in vitro conditions. Mural granulosa cells of small, medium, and preovulatory (PMSG) follicles also secreted CEEF in vitro; however, FSH or leutenizing hormone (LH) stimulation was essential for this secretion. ...Continue Reading

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Citations

Nov 8, 2003·Molecular and Cellular Endocrinology·Tetsuji OkazakiMasayuki Shimada
Mar 30, 2011·Human Reproduction Update·Maurizio ZuccottiSilvia Garagna
Jun 18, 2003·Reproductive Biology and Endocrinology : RB&E·Pia LucidiMauro Mattioli
Jun 15, 2013·Theriogenology·Sh Rouhollahi VarnosfaderaniM H Nasr-Esfahani
Feb 27, 2008·Reproduction in Domestic Animals = Zuchthygiene·H S RameshJ P Ravindra
Jan 15, 2014·Molecular Reproduction and Development·Jianmin SuYong Zhang
Nov 13, 2015·Animal Science Journal = Nihon Chikusan Gakkaihō·Yuta MatsunoKoji Sugiura
Jan 29, 2008·Zygote : the Biology of Gametes and Early Embryos·P S P GuptaJ P Ravindra
Mar 10, 2012·Biology of Reproduction·Kun-Liang LiJing-He Tan
Jan 8, 2008·Human Reproduction Update·Robert B GilchristJeremy G Thompson
Dec 19, 2020·Molecular and Cellular Endocrinology·Fang-Ju WuChing-Wei Luo

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