Secretion of functional Renilla reniformis luciferase by mammalian cells

Gene
J LiuA Escher

Abstract

The soft coral Renilla reniformis luciferase enzyme is a monomeric soluble intracellular protein that is used increasingly as a marker of gene expression. Here the Renilla luciferase gene was engineered to encode a protein product secreted by mammalian cells. The 5' end of the Renilla luciferase gene was fused in frame with the 3' end of a short DNA sequence encoding the signal peptide from human interleukin-2 (IL-2) protein. This construct was cloned under transcriptional control of the cytomegalovirus (CMV) promoter in a mammalian expression vector. Simian COS-7 cells were transiently transfected with the construct, and light emission was measured from cell lysates and from cell culture media. The results of these experiments indicated that Renilla luciferase was secreted as a functional enzyme by mammalian cells. The advantages and disadvantages of secreted Renilla luciferase as a marker of gene expression in comparison to other secreted protein markers are discussed.

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Citations

Feb 8, 2000·Luminescence : the Journal of Biological and Chemical Luminescence·J LiuA Escher
Mar 6, 1999·Current Opinion in Microbiology·D R Soll, T Srikantha
Apr 5, 2008·Genome Biology·Robin KettelerBrian Seed
Jul 14, 2004·Proceedings of the National Academy of Sciences of the United States of America·Yi-Kai ChenTing-Fang Wang
Dec 25, 2004·The Journal of Gene Medicine·Lei ZhangA James Mixson
Mar 11, 2004·Cellular Microbiology·Timothy C DoyleChristopher H Contag
Feb 14, 2013·Journal of Cellular and Molecular Medicine·Santiago RouraAntoni Bayes-Genis
May 23, 2006·Analytical Biochemistry·Kazutoshi YamagishiYoshihiro Ohmiya
Sep 17, 2011·Biotechnology Advances·Bakhos A Tannous, Jian Teng
Nov 5, 1999·Protein Expression and Purification·S C Makrides
Jan 19, 2018·Annual Review of Biophysics·Julien Béthune, Felix T Wieland
Dec 1, 2007·The Journal of Biological Chemistry·Erin MillerJacques U Baenziger
Apr 12, 2019·Frontiers in Bioengineering and Biotechnology·Natasha C DaleKevin D G Pfleger
Dec 26, 2018·Animal Biotechnology·Mohamed I Husseiny, Sameh Soliman
Jul 7, 2019·Biotechnology Advances·Kritika GuptaPrajakta Dandekar

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