Secretion of interleukin-6 and prostaglandin E2 during uveal melanoma-monocyte in vitro interactions

Experimental Eye Research
Jonathan Cools-LartigueMiguel N Burnier

Abstract

Host-tumor interactions in uveal melanoma are not well understood. It is believed that the cytokine interleukin-6 and the lipid mediator autacoid prostaglandin E2 are involved in tumor growth, proliferation, tumor cell survival, and angiogenesis. These cytokines have been shown to be poor prognostic markers in uveal and cutaneous melanoma. In this study, we investigated the levels of interleukin-6 and prostaglandin E2 in monocyte and uveal melanoma conditioned medium. Five human uveal melanoma cell lines (92.1, MKT-BR, OCM-1, SP6.5 and UW-1), and one monocyte cell line (28SC) were seeded in 6 well plates at a concentration of 1 x 10(6)cells ml(-1). After 18 hr melanoma conditioned medium was placed on the monocyte cell line and monocyte conditioned medium was placed on each uveal melanoma cell line. Tumor cells and monocytes incubated in fresh medium after 18 hr were used as controls. Interleukin-6 and prostaglandin E2 levels were determined by immunoassays prior to media transfer and 6, 12, 24, and 36 hr thereafter. In the absence of conditioned medium, neither product showed baseline levels of expression. Interleukin-6 but not prostaglandin E2, which remained undetectable for the duration of the study, showed up-regulation of...Continue Reading

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Citations

Mar 16, 2012·Retina·Roman DunavoelgyiUrsula Schmidt-Erfurth
Feb 4, 2012·Journal of Innate Immunity·Inge H G Bronkhorst, Martine J Jager
Feb 10, 2007·Clinical Practice and Epidemiology in Mental Health : CP & EMH·Massimo Pasquini, Massimo Biondi

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