Secretion of interleukin-6 by human endometriotic cells and regulation by proinflammatory cytokines and sex steroids

Human Reproduction
A AkoumR Maheux

Abstract

Endometriosis is generally associated with an immunoinflammatory process that takes place in the peritoneal cavity of patients. Interleukin (IL)-6, a multifunctional cytokine involved in numerous immunological and proliferative processes, has been found at high concentrations in the peritoneal fluid of endometriosis patients. The purpose of this study was to investigate the ability of endometriotic cells to produce IL-y and to assess the regulation of its secretion by proinflammatory cytokines and sex steroids. Cultures of human endometriotic cells were exposed to different concentrations of cytokines and sex steroid hormones for varying periods of time. IL-6 secretion was measured using an enzyme-linked immunosorbent assay. Endometriotic cells spontaneously released IL-6 in culture. IL-1 beta and tumour necrosis factor (TNF)-alpha (0.1-100.0 ng/ ml) potentiated IL-y secretion in a time- and dose-dependent manner. Interferon-gamma (0.4-400 ng/ml) induced a dose-related increase in IL-6 secretion and showed a synergistic effect on that secretion in combination with TNF-alpha (10 ng/ ml). Either spontaneous or cytokine-induced IL-6 secretion was inhibited by progesterone (10(-8)-10(-5) M) and danazol (10(-6) M), whereas oestradio...Continue Reading

Citations

Jul 19, 2005·Journal of Assisted Reproduction and Genetics·Mohamed A BedaiwyAshok Agarwal
May 12, 2004·Fertility and Sterility·Eugene R Shippen, William J West
Jul 5, 2001·Fertility and Sterility·T HaradaN Terakawa
Mar 7, 2003·Fertility and Sterility·Thitikorn WanichkulNeil Sidell
Nov 18, 1998·Current Opinion in Obstetrics & Gynecology·D P Braun, W P Dmowski
Aug 23, 2002·Current Opinion in Rheumatology·Joan T Merrill
Nov 17, 2012·Neuroimmunomodulation·Julia ArnoldMaria Luisa Barcena de Arellano
Dec 4, 2003·Reproductive Biology and Endocrinology : RB&E·Cleophas M KyamaThomas M D'Hooghe
Jan 24, 2014·Mediators of Inflammation·Dominic BertschiMichael D Mueller
Oct 24, 2007·Gynecological Endocrinology : the Official Journal of the International Society of Gynecological Endocrinology·Francesca MiniciRosanna Apa
Jun 3, 2000·Gynecological Endocrinology : the Official Journal of the International Society of Gynecological Endocrinology·M SchmidtA E Schindler
Aug 28, 2012·Fertility and Sterility·Edurne Novella-MaestreAntonio Pellicer
Nov 26, 2011·Reproductive Biomedicine Online·Sebastiano CampoGiuseppe Benagiano
Sep 4, 2007·Fertility and Sterility·Henrik FalconerThomas M D'Hooghe
Jun 22, 2007·European Journal of Obstetrics, Gynecology, and Reproductive Biology·Essam El-Din R OthmanAyman Al-Hendy
Nov 20, 2004·American Journal of Reproductive Immunology : AJRI·Osamu YoshinoYuji Taketani
Jul 12, 2003·American Journal of Reproductive Immunology : AJRI·Ming-Yih Wu, Hong-Nerng Ho
Feb 3, 2006·American Journal of Reproductive Immunology : AJRI·Jehn-Hsiahn YangHong-Nerng Ho
Mar 6, 2009·American Journal of Reproductive Immunology : AJRI·Ayako MiyamotoNaoki Terakawa
Aug 11, 2007·The Journal of Obstetrics and Gynaecology Research·Emmanuel KaluAmolak Bansal
Mar 15, 2006·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Roberta B Ness, Francesmary Modugno
Sep 15, 2005·American Journal of Obstetrics and Gynecology·Andrea D ShieldsPeter G Napolitano
Jul 16, 2003·American Journal of Obstetrics and Gynecology·Roberta B Ness
Feb 16, 2015·Fertility and Sterility·Sun-Wei GuoXishi Liu
May 9, 2006·Gynecologic and Obstetric Investigation·Admir AgicDaniela Hornung
Apr 26, 2017·American Journal of Reproductive Immunology : AJRI·Kuei-Yang HsiaoMeng-Hsing Wu
Jun 28, 2005·Journal of the Society for Gynecologic Investigation·Manjula BhanooriSisinthy Shivaji

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