Secretion of lutropin and follitropin from transfected GH3 cells: evidence for separate secretory pathways

Molecular Endocrinology
M MuyanI Boime

Abstract

Although lutropin (LH) and follitropin (FSH) are synthesized in the same pituitary gonadotropes, their secretion patterns in response to several experimental paradigms are not the same. Previous studies showing differences in secretion kinetics and the magnitude of hormone release by secretagogues imply differences in mechanisms for the storage and release of these hormones. To examine the secretory fate of LH and FSH, the genes encoding the common alpha-subunit and the corresponding beta-subunits were transfected in rat somatotrope-derived GH3 cells, which contain regulated and constitutive secretory pathways. The use of a gene transfer/heterologous cell system avoids physiological variations and functional heterogeneity of gonadotropes. Pulse-labeling and subsequent chase experiments demonstrated that although one third of newly synthesized FSH enters a regulated pathway, the majority is released constitutively. This contrasts with LH, which is mainly secreted through a regulated pathway. Although stored LH and FSH are released from GH3 cells in response to both KCl and forskolin, the magnitude of FSH release by secretagogues is smaller than that of LH. In Chinese hamster ovary cells, which are devoid of regulated secretory p...Continue Reading

Citations

Jul 9, 2011·Reviews in Endocrine & Metabolic Disorders·George R Bousfield, James A Dias
Dec 19, 1995·Proceedings of the National Academy of Sciences of the United States of America·U B KaiserW W Chin
Nov 6, 2009·Endocrinology·Christopher A PearlIrving Boime
Jan 10, 2009·Molecular Endocrinology·Albina Jablonka-Shariff, Irving Boime
Apr 12, 2015·General and Comparative Endocrinology·Hila AsrafDavid Ben-Menahem
Feb 11, 2015·Molecular and Cellular Endocrinology·Viktor Y ButnevGeorge R Bousfield

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