PMID: 9178822May 16, 1997Paper

Secretion of N-(4-hydroxyphenyl) retinamide-retinol-binding protein from liver parenchymal cells: evidence for reduced affinity of the complex for transthyretin

International Journal of Cancer. Journal International Du Cancer
K B HolvenR Blomhoff

Abstract

The synthetic retinoid 4-HPR has been shown to markedly lower the plasma concentration of both retinol and RBP in rats and humans. We have studied the effect of 4-HPR on the secretion of retinol-RBP from liver cells in vivo and in vitro. In rats maintained with a normal diet, a vitamin A-deficient diet or a normal diet supplemented with 4-HPR, chylomicrons [3H]retinyl esters were rapidly cleared from the plasma. The secretion of chylomicron-derived [3H]retinol from tissues to the circulation, however, was different. In control rats, the lymph-derived [3H]retinol peaked after about 2 hr, whereas 4-HPR treatment effectively reduced this peak of [3H]retinol. Our results suggest that 4-HPR inhibits secretion of retinol-RBP from the liver. Therefore, we decided to study the effect of 4-HPR on the secretion of RBP using the human hepatoma cell line HepG2. Retinol and 4-HPR were found to induce the secretion of RBP. The medium from cells treated with 4-HPR was immunoprecipitated with antibodies against human RBP. HPLC analysis of the precipitated RBP revealed the presence of 4-HPR. When the medium from cells incubated with either 4-HPR or retinol was applied to a TTR affinity column, we found that RBP from cells incubated with 4-HPR h...Continue Reading

References

Jan 1, 1991·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·N RotmenszM G Di Mauro
Jan 1, 1986·Archives of Ophthalmology·M I Kaiser-KupferE G Gross
Nov 1, 1986·Clinical and Experimental Dermatology·T P KingstonJ Heckenlively
Sep 1, 1993·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·R BerniF Formelli
Sep 30, 1995·Annals of the New York Academy of Sciences·A CostaU Veronesi
Jul 1, 1996·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·F FormelliJ A Olson

❮ Previous
Next ❯

Citations

Aug 17, 1999·Cancer Treatment Reviews·E Ulukaya, E J Wood
Dec 6, 2007·Journal of Korean Medical Science·Eun Jung ChoiYeul Hong Kim
May 4, 2007·Annals of Nutrition & Metabolism·Florian J Schweigert
Jul 20, 2006·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·UNKNOWN Children's Oncology Group (CCG 09709)Patrick C Reynolds
Sep 28, 2004·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Maria Grazia VillaniFranca Formelli

❮ Previous
Next ❯

Related Concepts

Related Feeds

Carcinoma, Hepatocellular

Hepatocellular Carcinoma is a malignant cancer in liver epithelial cells. Discover the latest research on Hepatocellular Carcinoma here.

Adrenocortical Carcinoma

Hepatocellular carcinoma is the most common type of primary liver cancer and frequently occurs in individuals with chronic liver diseases like cirrhosis. Here is the latest research.