Secretion of toxin A from Pseudomonas aeruginosa PAO1, PAK, and PA103 by Escherichia coli.

Infection and Immunity
A N HamoodB H Iglewski

Abstract

The exotoxin A gene (toxA) from Pseudomonas aeruginosa PAO1 was expressed from the lac promoter in Escherichia coli, and the localization of the toxin A protein was determined. Throughout the growth cycle, the ADP-ribosyltransferase activity of toxin A was gradually reduced in the periplasm of E. coli, with no apparent degradation of the toxin A protein. This suggests the presence of an E. coli periplasmic factor that interferes with the ADP-ribosyltransferase activity in toxin A. Such an inactivating factor was found in the periplasmic extract from control E. coli cells. The processing of toxin A in E. coli was examined by pulse-chase immunoprecipitation experiments. Mature toxin was detected in both the periplasm and cytoplasm, whereas the membranes contained both mature and precursor forms. Toxin A precursor appears to be processed in both the cytoplasm and the periplasm of E. coli. Toxin A proteins from P. aeruginosa PAO1, PA103, and PAK were compared for their secretion in E. coli. Despite the differences in the amino acid sequences of their leader peptides, toxin A proteins from strains PAO1, PA103, and PAK were processed and secreted to the periplasm of E. coli.

References

Jun 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·B H Iglewski, D Kabat
Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Nov 24, 1979·Nucleic Acids Research·H C Birnboim, J Doly
Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Apr 1, 1977·Infection and Immunity·M L VasilB H Iglewski
Apr 1, 1989·Journal of Bacteriology·A N HamoodB H Iglewski
Nov 1, 1988·Journal of Bacteriology·E Kessler, M Safrin
Mar 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·K L Strauch, J Beckwith
May 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·G L GrayH L Heyneker
May 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·M SchwartzM Débarbouillé
Mar 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·R JahnP Greengard

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