Secretion of wound healing mediators by single and bi-layer skin substitutes

Cytotechnology
Manira MaarofRuszymah Bt Hj Idrus

Abstract

Limitations of current treatments for skin loss caused by major injuries leads to the use of skin substitutes. It is assumed that secretion of wound healing mediators by these skin substitutes plays a role in treating skin loss. In our previous study, single layer keratinocytes (SK), single layer fibroblast (SF) and bilayer (BL; containing keratinocytes and fibroblasts layers) skin substitutes were fabricated using fibrin that had shown potential to heal wounds in preclinical studies. This study aimed to quantify the secretion of wound healing mediators, and compare between single and bi-layer skin substitutes. Skin samples were digested to harvest fibroblasts and keratinocytes, and expanded to obtain sufficient cells for the construction of skin substitutes. Acellular fibrin (AF) construct was used as control. Substitutes i.e. AF, SK, SF and BL were cultured for 2 days, and culture supernatant was collected to analyze secretion of wound healing mediators via multiplex ELISA. Among 19 wound healing mediators tested, BL substitute secreted significantly higher amounts of CXCL1 and GCSF compared to SF and AF substitute but this was not significant with respect to SK substitute. The BL substitute also secreted significantly higher...Continue Reading

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Citations

Mar 6, 2018·Military Medical Research·Chinedu Cletus UdeMuhamad Bin Abu Bakar
Oct 22, 2018·The Protein Journal·Manira MaarofShiplu Roy Chowdhury
Dec 14, 2018·Drug Delivery and Translational Research·Manira MaarofShiplu Roy Chowdhury
Jul 15, 2017·Scientific Reports·Elin KindstedtPernilla Lundberg
Jul 28, 2017·Cell and Tissue Banking·Jia Xian LawBinti Haji Idrus Ruszymah
Aug 10, 2017·Tissue Engineering and Regenerative Medicine·Jia Xian LawRuszymah Idrus
Nov 29, 2020·Journal of Materials Science. Materials in Medicine·Kanokaon BenchaprathanphornKwanchanok Viravaidya-Pasuwat

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