Secretion, purification, and characterization of a recombinant Aspergillus oryzae tannase in Pichia pastoris

Protein Expression and Purification
Xiaofen ZhongAnlong Xu

Abstract

Tannase (tannin acyl hydrolase) is an industrially important enzyme produced by a large number of fungi, which hydrolyzes the ester and depside bonds of gallotannins and gallic acid esters. In the present work, a tannase from Aspergillus oryzae has been cloned and expressed in Pichia pastoris. The catalytic activity of the recombinant enzyme was assayed. A secretory form of enzyme was made with the aid of Saccharomyces cerevisiae alpha-factor, and a simple procedure purification protocol yielded tannase in pure form. The productivity of secreted tannase achieved 7000 IU/L by fed-batch culture. Recombinant tannase had a molecular mass of 90 kDa, which consisted of two kinds of subunits linked by a disulfide bond(s). Our study is the first report on the heterologous expression of tannase suggesting that the P. pastoris system represents an attractive means of generating large quantities of tannase for both research and industrial purpose.

References

Jan 1, 1990·Annals of the New York Academy of Sciences·R A BrierleyR S Siegel
Aug 1, 1993·Bio/technology·J M CreggW C Raschke

❮ Previous
Next ❯

Citations

May 29, 2007·Applied Microbiology and Biotechnology·Cristóbal N AguilarJuan C Contreras-Esquivel
Sep 6, 2007·Applied Microbiology and Biotechnology·Monika Kasieczka-BurneckaMarianna Turkiewicz
May 12, 2011·Applied Microbiology and Biotechnology·Erik BöerGotthard Kunze
Sep 25, 2012·Applied Microbiology and Biotechnology·Takuya KosekiYoshihito Shiono
Aug 21, 2013·Applied Biochemistry and Biotechnology·E Zakipour-MolkabadiM H Azizi
May 27, 2014·Journal of Bioscience and Bioengineering·Toshiyuki MizunoTakuya Koseki
Mar 16, 2011·Journal of Bioscience and Bioengineering·Shailendra RaghuwanshiRajendra Kumar Saxena
Jun 16, 2007·Bioresource Technology·Shashi SharmaRajendra Kumar Saxena
Jun 21, 2005·Bioresource Technology·Jogeswar Satchidananda PurohitRintu Banerjee
Sep 24, 2011·Enzyme Research·Luis V Rodríguez-DuránCristóbal N Aguilar
Jan 13, 2015·Molecular Biotechnology·José Antonio Fuentes-GaribayJosé María Viader-Salvadó
Feb 18, 2015·Journal of Biotechnology·Sebastian C SpohnerPeter Czermak
Apr 1, 2014·Preparative Biochemistry & Biotechnology·Shuai ZhangYong Cao
Feb 14, 2006·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·R B CraveiroJ B Pesquero
Oct 27, 2015·Biotechnology and Applied Biochemistry·Venkatramanan VaradharajanPriyadharshini Chandrasekar
Nov 22, 2018·Applied Microbiology and Biotechnology·Blanca de Las RivasRosario Muñoz
Feb 11, 2005·Yeast·Sue Macauley-PatrickLinda M Harvey
Feb 28, 2019·International Microbiology : the Official Journal of the Spanish Society for Microbiology·Sunny DhimanAbhishek Kumar Singh
Jul 16, 2009·Journal of Agricultural and Food Chemistry·José Antonio CurielRosario Muñoz

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.