Secretome Analysis and In Planta Expression of Salivary Proteins Identify Candidate Effectors from the Brown Planthopper Nilaparvata lugens

Molecular Plant-microbe Interactions : MPMI
Weiwei RaoGuangcun He

Abstract

The brown planthopper (BPH), Nilaparvata lugens (Stål), is a phloem sap-feeding insect. During feeding on rice plants, BPH secretes salivary proteins with potential effector functions, which may play a critical role in the plant-insect interactions. However, a limited number of BPH effector proteins have been identified to date. Here, we sequenced the salivary gland transcriptomes of five BPH populations and subsequently established a N. lugens secretome consisting of 1,140 protein-encoding genes. Secretome analysis revealed the presence of both conserved and rapidly evolving salivary proteins. A screen for potential effectors that elicit responses in the plant was performed via the transient expression analysis of 64 BPH salivary proteins in Nicotiana benthamiana leaves and rice protoplasts. The salivary proteins Nl12, Nl16, Nl28, and Nl43 induced cell death, whereas Nl40 induced chlorosis and Nl32 induced a dwarf phenotype in N. benthamiana, indicating effector properties of these proteins. Ectopic expression of the six salivary proteins in N. benthamiana upregulated expression of defense-related genes and callose deposition. Tissue expression analysis showed a higher expression level of the six candidate effectors in salivar...Continue Reading

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Citations

Jan 23, 2019·Archives of Insect Biochemistry and Physiology·Hai-Jian HuangXiao-Yue Hong
Apr 9, 2020·Scientific Reports·Inaiara de Souza PachecoMarcos Antonio Machado
Nov 24, 2020·Current Opinion in Insect Science·Xiaohong ZhengGuangcun He
May 24, 2021·Current Opinion in Biotechnology·Philippe Reymond
Aug 8, 2021·Biochemical and Biophysical Research Communications·Zhen WangHansong Dong

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