Secretome analysis of rice suspension-cultured cells infected by Xanthomonas oryzae pv.oryza (Xoo)

Proteome Science
Xian ChenJianping Chen

Abstract

Rice bacterial blight (BB) caused by Xanthomonas oryzae pv.oryzae (Xoo) is one of the most devastating bacterial diseases in rice-growing regions worldwide. The rice-Xoo interaction is a classical model for studying the interaction between plants and pathogens. Secreted proteins play important roles in plant-bacterial interactions, but are poorly studied in the rice-Xoo system. Rice cv. Nipponbare is highly susceptible to Xoo. Here, we used two-dimensional difference gel electrophoresis (2D-DIGE) coupled with MALDI-TOF/TOF mass spectrometry (MS), to investigate secreted proteins in Nipponbare embryo cell suspension culture infected by Xoo. A total of 32 protein spots changed significantly (p < 0.05) by more than 1.5 fold in gel intensity after Xoo inoculation, and were identified by MS. They represent protein products of 11 unique genes, seven from rice and four from Xoo. Of the rice proteins, six up-regulated proteins are involved in cell wall modification, the TCA cycle, glycolysis and redox, while a down-regulated protein, CHIT16, is involved in plant defense. Quantitative Real-Time PCR showed that transcript levels were not correlated with secreted protein levels. Of the Xoo proteins, three of them were possibly located in ...Continue Reading

References

Dec 1, 1994·Plant Molecular Biology·E D SchmidtS C de Vries
Nov 10, 2001·Annual Review of Phytopathology·S H HulbertQ Sun
Jun 18, 2002·Infection and Immunity·Dirk BumannPeter R Jungblut
Apr 26, 2003·The Plant Journal : for Cell and Molecular Biology·Ana Caño-DelgadoMichael Bevan
Nov 11, 2005·Proteomics·Stephen ChivasaAntoni R Slabas
Dec 1, 2005·Molecular & Cellular Proteomics : MCP·Shun-Ping YanWei-Ning Sun
Dec 6, 2005·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Zhaohui ChuShiping Wang
Apr 12, 2007·Expert Review of Proteomics·Yetrib Hathout
Sep 13, 2007·Molecular & Cellular Proteomics : MCP·Zhiping DengZhi-Yong Wang
May 22, 2009·Plant Physiology·Frank F White, Bing Yang
Sep 22, 2009·The Plant Journal : for Cell and Molecular Biology·Ming LiYihua Zhou
Dec 3, 2009·Proteomics·Ganesh Kumar AgrawalRandeep Rakwal
Oct 4, 2011·Journal of Biotechnology·Valérie VerdierJan E Leach
Aug 1, 1999·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Y NishizawaT Hibi
Jun 7, 2014·Annual Review of Phytopathology·Wende LiuGuo-Liang Wang

❮ Previous
Next ❯

Methods Mentioned

BETA
electrophoresis
protein ladder
transgenic
PCR

Software Mentioned

MASCOT
PSORT
GPS
SecretomeP
Primer Premier
SignalP
Decyder 2D
BLAST

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.