Secretome of gingival epithelium in response to subgingival biofilms

Molecular Oral Microbiology
N BostanciG N Belibasakis

Abstract

Periodontitis is the chronic inflammatory destruction of periodontal tissues as a result of bacterial biofilm formation on the tooth surface. Proteins secreted by the gingival epithelium challenged by subgingival biofilms represent an important initial response for periodontal inflammation. The aim of this in vitro study was to characterize the whole secreted proteome of gingival epithelial tissue challenged by subgingival biofilms, and to evaluate the differential effects of the presence of the red-complex species in the biofilm. Multi-layered human gingival epithelial cultures were challenged with a 10-species in vitro biofilm model or its seven-species variant excluding the red complex. Liquid chromatography-tandem mass spectrometry for label-free quantitative proteomics was applied to identify and quantify the secreted epithelial proteins in the culture supernatant. A total of 192 proteins were identified and quantified. The biofilm challenge resulted in more secreted proteins being downregulated than upregulated. Even so, presence of the red complex in the biofilm was responsible for much of this downregulatory effect. Over 24 h, the upregulated biological processes were associated with inflammation and apoptosis, whereas ...Continue Reading

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Citations

Aug 26, 2015·Virulence·G N BelibasakisNagihan Bostanci
Nov 5, 2016·Diagnostic Microbiology and Infectious Disease·Janaína de Cássia Orlandi SardiMaria José Soares Mendes-Giannini
Dec 21, 2016·Proteomics·Nagihan Bostanci, Kai Bao
Oct 14, 2017·Expert Review of Clinical Immunology·Courtney P RudickDevendra K Agrawal
Oct 31, 2017·NPJ Biofilms and Microbiomes·Samir A ShahPurnima S Kumar
Sep 12, 2017·Journal of Dental Research·J K BuskermolenS Gibbs
Mar 7, 2019·Frontiers in Immunology·Sabine Groeger, Joerg Meyle
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Dec 19, 2019·Periodontology 2000·Trang NguyenYvonne L Kapila
Oct 9, 2020·Critical Reviews in Microbiology·Chaminda J SeneviratneGordon Ramage
Dec 10, 2020·Journal of Medical Microbiology·Ioannis FragkioudakisDanae Anastasia Apatzidou
Aug 11, 2020·Journal of Clinical Periodontology·Nagihan BostanciRodrigo Lopez
Mar 21, 2021·Scientific Reports·Nagihan BostanciMichal Karpíšek

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