Secretome of Senescent Adipose-Derived Mesenchymal Stem Cells Negatively Regulates Angiogenesis

International Journal of Molecular Sciences
Andrey RatushnyyLudmila Buravkova

Abstract

Nowadays, paracrine regulation is considered as a major tool of mesenchymal stem cell (MSC) involvement in tissue repair and renewal in adults. Aging results in alteration of tissue homeostasis including neovascularization. In this study, we examined the influence of replicative senescence on the angiogenic potential of adipose-derived MSCs (ASCs). Angiogenic activity of conditioned medium (CM) from senescent and "young" ASCs was evaluated in chorioallantoic membrane (CAM) assay in ovo using Japanese quail embryos. Also, the formation of capillary-like tubes by human umbilical vein endothelial cells (HUVECs) in 3D basement membrane matrix ''Matrigel'' and HUVEC migration capacity were analyzed. Multiplex, dot-blot and gene expression analysis were performed to characterize transcription and production of about 100 angiogenesis-associated proteins. The results point to decreased angiogenic potential of senescent ASC secretome in ovo. A number of angiogenesis-associated proteins demonstrated elevation in CM after long-term cultivation. Meanwhile, VEGF (key positive regulator of angiogenesis) did not change transcription level and concentration in CM. Increasing both pro- (FGF-2, uPA, IL-6, IL-8 etc.) and antiangiogenic (IL-4, IP-...Continue Reading

References

Sep 7, 1994·Journal of the National Cancer Institute·R PiliA Passaniti
Sep 26, 1995·Proceedings of the National Academy of Sciences of the United States of America·G P DimriO Pereira-Smith
Apr 2, 1999·Science·M F PittengerD R Marshak
Feb 15, 2001·Biochemical Pharmacology·S LiekensJ Neyts
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Apr 30, 2003·The Journal of Biological Chemistry·Alfonso LuqueM Luisa Iruela-Arispe
Jul 31, 2003·Cell·Dong-Wan SeoWilliam G Stetler-Stevenson
Oct 21, 2005·Cytotherapy·E M HorwitzUNKNOWN International Society for Cellular Therapy
Apr 4, 2006·Nature Medicine·Yoshinori MiyaharaHidezo Mori
Jun 23, 2006·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Mi Hyang MoonJin Sup Jung
Aug 2, 2006·The Journal of Biological Chemistry·Jean-Philippe CoppéChristian M Beauséjour
Sep 27, 2006·Journal of Cellular Physiology·Mikhail V Blagosklonny
Mar 21, 2007·World Journal of Surgery·Areck A Ucuzian, Howard P Greisler
Mar 27, 2007·Growth Factors·Anne-Marie MaurerZhong Chao Han
May 15, 2007·Circulation Research·Jeffrey M GimbleBruce A Bunnell
Aug 2, 2007·Nature Reviews. Molecular Cell Biology·Judith Campisi, Fabrizio d'Adda di Fagagna
Nov 6, 2007·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Yeon KimJin Jung
Nov 1, 2008·Arteriosclerosis, Thrombosis, and Vascular Biology·Kazuhisa KondoToyoaki Murohara
Jan 10, 2009·Nature Reviews. Cancer·Thomas Kuilman, Daniel S Peeper
Jul 25, 2009·Aging Cell·Chunfang WangThomas von Zglinicki
Oct 16, 2009·Histochemistry and Cell Biology·Kohtaro MiyazawaHisashi Aso
Dec 10, 2009·Journal of Cardiovascular Medicine·Rosalinda Madonna, Raffaele De Caterina
Dec 19, 2009·Regenerative Medicine·Priya R Baraniak, Todd C McDevitt
Jan 19, 2010·Annual Review of Pathology·Jean-Philippe CoppéJudith Campisi
Jul 6, 2010·Nucleic Acids Research·Kai WangHakon Hakonarson
Jan 20, 2011·Journal of Translational Medicine·Anastasia EfimenkoAlexandra Stolzing
May 20, 2011·Nature·Peter Carmeliet, Rakesh K Jain
Jan 19, 2012·Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission·H E GruberE N Hanley
Apr 28, 2012·Circulation Research·Johanna Lähteenvuo, Anthony Rosenzweig
Oct 17, 2012·Molecular Therapy : the Journal of the American Society of Gene Therapy·Sandra De BarrosValérie Planat-Bénard
Nov 16, 2013·Experimental & Molecular Medicine·Matthew B MurphyArnold I Caplan
Dec 7, 2013·BioMed Research International·Marta BarbaWanda Lattanzi
Jan 6, 2015·Apoptosis : an International Journal on Programmed Cell Death·Jian Hua Qi, Bela Anand-Apte
Apr 26, 2016·Molecular Medicine Reports·Yajun GuXiaochun Sun
Jul 19, 2016·International Journal of Stem Cells·Diana LegzdinaUldis Berzins

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Citations

Jun 24, 2020·International Journal of Molecular Sciences·Anthony M BattramBeatriz Martín-Antonio
Jan 29, 2021·World Journal of Stem Cells·Alberto González-GonzálezFlor M Pérez-Campo
Nov 18, 2020·Biochemistry. Biokhimii︠a︡·A Y RatushnyyL B Buravkova
Mar 25, 2021·Journal of Cellular and Molecular Medicine·Kun LiuGuang Han

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Methods Mentioned

BETA
flow cytometry
Profiler
Assay
PCR

Software Mentioned

Sigma ScanPro
SPSS
Elements
Pro Plus
AngioQuant
Image Lab
NIS
Image

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