Secretome profile of mouse oocytes after activation using mass spectrum.

Journal of Assisted Reproduction and Genetics
Qiuping PengYanping Kuang

Abstract

Mammalian oocytes undergo a cortical reaction after fertilization, releasing cortical granules and other proteins into the perivitelline space and inhibiting polyspermy. Few studies have evaluated the biological functions and properties of these proteins. We investigated mouse oocytes in which the zona pellucida (ZP) was present (ZP-intact group) or absent (ZP-free group). After being activated by Srcl2, secreted proteins are collected from mouse oocytes. Mass spectrometry analysis was performed that identified proteins such as Ldhb, PADi6, Uchl1, Pebp1, Alb, Hsp90aa1, Prss1, trypsinogen 7, trypsin 4, trypsin 10, Sod1, Zp1, Zp2, Zp3, Akap8, Npm2, Pkm2 and Ppia in the ZP-free group. Proteins such as Ldhb, Uchl1, Prss1, trypsin 10, trypsinogen 7, and Ast1 were identified in the ZP-intact groups. The expression of some proteins, including Ldhb, Alb and Sod1, were initially detected following oocyte activation. The finding of four trypsin subtypes, such as Prss1, further support previous observations. Studies investigating the physiological functions and properties of these proteins are ongoing. Research on these cortical proteins provides a theoretical basis for understanding polyspermy inhibition at the level of ZP and gives tech...Continue Reading

References

May 1, 1992·Molecular Reproduction and Development·X ZhangD T Armstrong
Dec 28, 1999·Biology of Reproduction·M Lane, D K Gardner
Oct 13, 2001·Molecular Reproduction and Development·R J Muñoz-GoteraA Mújica
Jan 25, 2002·European Journal of Immunology·Beatrice FuzziOlavio R Baricordi
Jun 20, 2003·Reproduction : the Official Journal of the Society for the Study of Fertility·K IwahashiK Akagawa
Nov 14, 2003·Reproductive Biology and Endocrinology : RB&E·Min LiuPrue Talbot
Aug 6, 2005·Protein Science : a Publication of the Protein Society·Ying-Xia ZhangYun Zhang
Sep 6, 2006·Fertility and Sterility·Mandy G Katz-JaffeDavid K Gardner
Oct 21, 2006·Proceedings of the National Academy of Sciences of the United States of America·Roger A O'NeillPeter B Vander Horn
Oct 31, 2006·The American Journal of Pathology·Satoshi SekiguchiYasuhiro Yoshikawa
Jun 26, 2007·Molecular and Cellular Endocrinology·G EspositoJ A Gossen
Dec 19, 2008·PloS One·Monica BorgattiRoberto Gambari
Feb 19, 2010·Biology of Reproduction·Andrej SusorMichal Kubelka
Jun 24, 2010·Reproductive Biology and Endocrinology : RB&E·Amanda J BeardsleyChris O'Neill

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Citations

May 8, 2014·BioMed Research International·Irma Virant-Klun, Jeroen Krijgsveld
Feb 13, 2016·Proceedings of the National Academy of Sciences of the United States of America·Hakan CakmakMarco Conti
Sep 17, 2013·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Eusebio S PiresJohn C Herr
Sep 16, 2017·Molecular Human Reproduction·Hagen KörschgenWalter Stöcker
Jan 27, 2019·Scientific Reports·Konstantin KarmilinWalter Stöcker
Apr 11, 2020·Biogerontology·Karolin YanarUfuk Çakatay

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