Secretory apparatus assessed by analysis of pancreatic secretory stress protein expression in a rat model of chronic pancreatitis

Cell and Tissue Research
Severin MeiliDaniel Bimmler

Abstract

Secretory stress proteins (SSP) are a family of proteins including isoforms of pancreatitis-associated protein (PAP) and pancreatic stone protein (PSP/reg). In vitro exposure to trypsin results in the formation of insoluble fibrillar structures. SSP are constitutively secreted into pancreatic juice at low levels. The WBN/Kob rat is a model for chronic pancreatitis, displaying focal inflammation, destruction of the parenchyma and changes in the architecture of the acinar cell; the synthesis and secretion of SSP are also increased. We have investigated the secretory apparatus by SSP immunohistochemistry at the light- and electron-microscopical (EM) levels. Immunocytochemistry of PSP/reg in Wistar control rats reveals low levels, with individual acinar cells exhibiting high immunoreactivity in zymogen granules. PAP is not detectable. In the WBN/Kob rat, PSP/reg and PAP immunoreactivity is markedly increased. Double immunofluorescence for PSP/reg and PAP I or II demonstrates that these proteins colocalize to the same cell. Acinar cells change their secretory architecture by fusion of zymogen granules and elongation of the fused organelles. The immunogold technique has demonstrated an increase of SSP in zymogen granules in WBN/Kob r...Continue Reading

Citations

May 17, 2006·BMC Gastroenterology·Oxana NorkinaRobert C De Lisle
Apr 27, 2010·Cancer Genetics and Cytogenetics·Ying GuoPeilin Huang
Sep 24, 2010·European Journal of Clinical Investigation·Ling LiMax G Bachem
Dec 12, 2003·World Journal of Gastroenterology : WJG·Yu-Wei ZhangMao-De Lai
Feb 11, 2020·Pancreatology : Official Journal of the International Association of Pancreatology (IAP) ... [et Al.]·Rolf Graf

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