PMID: 8589421Jan 1, 1996Paper

Secretory granule proteins from the subventral esophageal glands of the potato cyst nematode identified by monoclonal antibodies to a protein fraction from second-stage juveniles

Molecular Plant-microbe Interactions : MPMI
J M de BoerArjen Schots

Abstract

Sodium dodecyl sulfate-extracted proteins from second-stage juveniles (J2) of the potato cyst nematode Globodera rostochiensis were fractionated by preparative continuous flow electrophoresis, and monoclonal antibodies (MAbs) were raised against the 38- to 40.5-kDa protein fraction. Screening of the hybridoma culture fluids by immunofluorescence microscopy of J2 resulted in the identification of 12 MAbs that bound specifically to the subventral esophageal glands. On Western blots of J2 these MAbs identified four protein bands with apparent molecular masses of 30, 31, 39, and 49 kDa. Immunoelectron microscopy with one of these MAbs showed an intense labeling of the electron dense core of the secretory granules in the subventral gland cells of J2. It is concluded that one or more of these proteins are localized within these secretory granules. Immunofluorescence microscopy of J2 from other plant parasitic nematode species showed that most of these MAbs also bind to the subventral glands of G. pallida and G. tabacum but not of Heterodera schachtii, H. glycines, Meloidogyne incognita, or M. hapla.

Citations

Nov 5, 1999·Biochimica Et Biophysica Acta·G TokudaH Noda
Jan 13, 1999·Molecular Plant-microbe Interactions : MPMI·X WangE Davis
Oct 1, 1996·The Plant Cell·V M Williamson, R S Hussey
Nov 10, 2001·Annual Review of Phytopathology·Eric L DavisPierre Abad
Jun 6, 1998·Proceedings of the National Academy of Sciences of the United States of America·G SmantJ Bakker
Jun 6, 1998·Proceedings of the National Academy of Sciences of the United States of America·N T Keen, P A Roberts
May 15, 2012·Annual Review of Phytopathology·Melissa G MitchumEric L Davis
Mar 27, 2010·Annual Review of Phytopathology·Richard S Hussey
Nov 14, 1997·FEBS Letters·A SchoutenA Schots
May 23, 2013·The New Phytologist·Melissa G MitchumEric L Davis
Jul 3, 2004·Current Opinion in Plant Biology·David McK Bird
May 18, 2004·Gene·Bartel VanholmeGodelieve Gheysen
Jul 14, 2000·Nature·H PopeijusG Smant

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